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MEGACD40L® Protein (soluble) (human), (recomb

更新时间:2022-01-28

简要描述:

MEGACD40L® is a high activity protein in which two trimeric CD40 ligand molecules are artificially linked via the collagen domain of Adiponectin/ACRP30/AdipoQ. This protein very effectively ……

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  • Highly active MEGACD40L® oligomer mimics in vivo membrane-assisted CD40L aggregation and stimulation without the need for enhancers

  • High purity with low endotoxin levels

  • Experienced manufacturing results in consistent and dependable lot-to-lot performance

  • Optimized formulation for improved stability and enhanced activity

MEGACD40L® is a high activity protein in which two trimeric CD40 ligand molecules are artificially linked via the collagen domain of Adiponectin/ACRP30/AdipoQ. This protein very effectively simulates the natural membrane-assisted aggregation of CD40L in vivo.  It provides a simple and equally potent alternative to [CD40L+enhancer] combinations (Prod. No. ALX-850-064).

Product Details

Alternative Name:CD40L:ACRP30headless, CD154:ACRP30headless, TNFSF 5:ACRP30headless, gp39:ACRP30headless

MW:~38kDa

Source:Produced in CHO cells. The extracellular domain of human CD40L (CD154) (aa 116-261) is fused at the N-terminus to mouse ACRP30headless (aa 18-111) and a FLAG®-tag.

UniProt ID:P29965 (human CD40L), Q60994 (mouse Adiponectin)

Concentration:0.1mg/ml after reconstitution.

Formulation:Lyophilized. Contains PBS.

Purity:≥90% (SDS-PAGE)

Purity Detail:Purified by multi-step chromatography.

Endotoxin Content:≤0.01EU/µg purified protein (LAL test; Cape Cod Associates).

Biological Activity:Binds to human CD40. Induces B cells activation (as demonstrated by dose-dependent upregulation of CD86).

Application Notes:Can be used to mediate immune and inflammatory responses, as a protein tool for pre-clinical studies in immunotherapy, to monitor patients undergoing chemotherapy and to mimic autoimmune phenotypes in vivo.

Reconstitution:Reconstitute with 100µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum.

Shipping:Shipped on Blue Ice

Long Term Storage:-20°C

Use/Stability:Stable for at least 6 months after receipt when stored at -20°C.

Handling:Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.

Technical Info/Product Notes:FLAG is a registered trademark of Sigma-Aldrich Co.

Regulatory Status:RUO - Research Use Only

MEGACD40L® Protein (soluble) (human), (recombinant) Flow Cytometry
B cell lymphocyte activation by MEGACD40L® (Prod. No. ALX-522-110). Method: Peripheral Blood Mononuclear Cells (PBMCs) were incubated at 37°C, 5% CO2 for 48 hours in 48 well plates (1 x 106 cells/well). Each well contained 200µl serum free test media with serially diluted MEGACD40L®. After treatment, cells were washed and dual-stained with mouse anti-human CD19–PE and mouse anti-human CD86–APC and analyzed by flow cytometry. The data are presented as the percent of CD19 positive B cells that co-stain as CD86 positive, at each concentration of MEGACD40L®.
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MEGACD40L® Protein (soluble) (human), (recombinant) Flow CytometryMEGACD40L® Protein (soluble) (human), (recombinant) Fig1MEGACD40L® Protein (soluble) (human), (recombinant) Flow CytometryMEGACD40L® Protein (soluble) (human), (recombinant) Fig4MEGACD40L® Protein (soluble) (human), (recombinant) Fig5


Product Literature References

Inverse Regulation of Confluence-Dependent ADAMTS13 and von Willebrand Factor Expression in Human Endothelial Cells: M. Popa, et al.; Thromb. Haemost. (2021), Abstract;
Quantitative assessment of NFκB transcription factor activity: T.J. Hunter, et al.; J. Immunol. Methods 2021, 112954 (2021), Abstract;
Renal Fibrosis Is Significantly Attenuated Following Targeted Disruption of Cd40 in Experimental Renal Ischemia: S. Zhang, et al.; J. Am. Heart Assoc. 9, e014072 (2020), Abstract; Full Text
C1s Inhibition by BIVV009 (Sutimlimab) Prevents Complement-Enhanced Activation of Autoimmune Human B Cells In Vitro: P.A. Nikitin, et al.; J. Immunol. 202, 1200 (2019), Abstract; Full Text
CRISPR/Cas9 Screens Reveal Multiple Layers of B cell CD40 Regulation: C. Jiang, et al.; Cell Rep. 28, 1307 (2019), Abstract; Full Text
Epstein-Barr-Virus-Induced One-Carbon Metabolism Drives B Cell Transformation: L.W. Wang, et al.; Cell Metab. 30, 539 (2019), Abstract; Full Text
Type 1-programmed dendritic cells drive antigen-specific latency reversal and immune elimination of persistent HIV-1: J. Kristoff, et al.; EBioMedicine 43, 295 (2019), Abstract; Full Text
CD40L and TNF both activate the classical NF-κB pathway, which is not required for the CD40L induced alternative pathway in endothelial cells: J. Seigner, et al.; Biochem. Biophys. Res. Commun. 495, 1389 (2018), Abstract;
CD40L mediated alternative NFκB-signaling induces resistance to BCR-inhibitors in patients with mantle cell lymphoma: H. Rauert-Wunderlich, et al.; Cell Death Dis. 9, 86 (2018), Abstract; Full Text
Characterisation of anifrolumab, a fully human anti-interferon receptor antagonist antibody for the treatment of systemic lupus erythematosus: J.M. Riggs, et al.; Lupus Sci. Med. 5, e000261 (2018), Abstract; Full Text
Engineering protein-secreting plasma cells by homology-directed repair in primary human B cells: K.L. Hung, et al.; Mol. Ther. 26, 456 (2018), Abstract;
High PD-L1/CD86 MFI ratio and IL-10 secretion characterize human regulatory dendritic cells generated for clinical testing in organ transplantation: A.F. Zahorchak, et al.; Cell. Immunol. 323, 9 (2018), Abstract;
In vitro antineoplastic effects of auranofin in canine lymphoma cells: H. Zhang, et al.; BMC Cancer 18, 522 (2018), Abstract; Full Text
Arginine methylation catalyzed by PRMT1 is required for B cell activation and differentiation: S. Infantino, et al.; Nat. Commun. 8, 891 (2017), Application(s): B-cell activation, Abstract; Full Text
B-cell activation with CD40L or CpG measures the function of B-cell subsets and identifies specific defects in immunodeficient patients: E. Marasco, et al.; Eur. J. Immunol. 47, 131 (2017), Application(s): Human PBMC culture, Abstract; Full Text
CD40 signaling in Graves disease is mediated through canonical and noncanonical thyroidal nuclear factor κB activation: H.J. Lee, et al.; Endocrinology 158, 410 (2017), Abstract; Full Text


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