更新时间:2022-03-03
The FLuc mRNA will express a luciferase protein, originally isolated from the firefly, Photinus pyralis. FLuc is commonly used in mammalian cell culture to measure both gene expression and cell viabi…
Description
The FLuc mRNA will express a luciferase protein, originally isolated from the firefly, Photinus pyralis. FLuc is commonly used in mammalian cell culture to measure both gene expression and cell viability. It emits bioluminescence in the presence of the substrate, luciferin.
This mRNA is capped using CleanCap, TriLink's proprietary co-transciptional capping method, which results in the naturally occuring Cap 1 structure with high capping efficiency. It is polyadenylated, modified with 5-methoxyuridine and optimized for mammalian systems. It mimics a fully processed mature mRNA.
Product Details
Catalog NoL-7202
PurityPasses Agarose Gel Mobility
Length1929 nucleotides
Base CompositionFully substituted with 5-Methoxy-U
Concentration1.0 mg/mL
Buffer1 mM Sodium Citrate pH 6.4
Conversion Factor40 µg/OD260
Recommended StorageAt or below -40°C
ApplicationReporter Genes
CapAG Start, Cap 1, CleanCap
Other Name(s)CleanCap®Firefly Luciferase mRNA (5-methoxyuridine)