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BioXcell代理欣博盛生物:InVivoPlus anti-mouse PD-L1 (B7-H1)

更新时间:2023-05-18   点击次数:318次

BioXcell热销产品推荐--InVivoPlus anti-mouse PD-L1 (B7-H1)


产品描述:

BioXcell InVivoPlus anti-mouse PD-L1 10F.9G2单克隆抗体与小鼠PD-L1(也称为B7-H1或CD274)反应。PD-L1是属于Ig超家族的B7家族的I型跨膜蛋白,分子量为40kDa。PD-L1在T淋巴细胞、B淋巴细胞、NK细胞、树突状细胞以及IFNγ刺激的单核细胞、上皮细胞和内皮细胞上表达。PD-L1与CD4和CD8胸腺细胞以及活化的T和B淋巴细胞和骨髓细胞上的受体PD-1结合。PD-L1与PD-1的结合导致抑制TCR介导的T细胞增殖和细胞因子产生。PD-L1被认为在肿瘤免疫逃逸中起着重要作用。诱导的PD-L1表达在许多肿瘤中很常见,并导致肿瘤细胞对CD8 T细胞介导的裂解的抗性增加。在黑色素瘤的小鼠模型中,可以通过用阻断PD-L1和PD-1之间相互作用的抗体进行治疗,暂时抑制肿瘤生长。10F.9G2抗体已被证明可以阻断PD-L1和PD-1之间以及PD-L1和B7-1之间的相互作用(CD80)。

BioXcell热销产品推荐--InVivoPlus anti-mouse PD-L1 (B7-H1) 

产品详情:

产品名称

InVivoPlus anti-mouse PD-L1 (B7-H1)

产品货号

BP0101

产品规格

5/25/50/100mg

反应种属

Mouse

克隆号

10F.9G2

同种型

Rat IgG2b, κ

免疫原

Mouse CD274

实验应用

in vivo PD-L1 blockade
Immunofluorescence
Immunohistochemistry (frozen)
Flow cytometry
Western blot

产品形式

PBS, pH 6.5,Contains no stabilizers or preservatives

纯度

>95%, Determined by SDS-PAGE

聚合

<5%, Determined by SEC

无菌处理

0.2 µm filtration

纯化方式

Protein G

RRID

AB_10949073

分子量

150 kDa

小鼠病原检测

Ectromelia/Mousepox Virus: Negative

Hantavirus: Negative

K Virus: Negative

Lactate Dehydrogenase-Elevating Virus: Negative

Lymphocytic Choriomeningitis virus: Negative

Mouse Adenovirus: Negative

Mouse Cytomegalovirus: Negative

Mouse Hepatitis Virus: Negative

Mouse Minute Virus: Negative

Mouse Norovirus: Negative

Mouse Parvovirus: Negative

Mouse Rotavirus: Negative

Mycoplasma Pulmonis: Negative

Pneumonia Virus of Mice: Negative

Polyoma Virus: Negative

Reovirus Screen: Negative

Sendai Virus: Negative

Theiler’s Murine Encephalomyelitis: Negative

保存条件

抗体原液保存在4°C,不能冷冻保存。

推荐同型对照

InVivoPlus rat IgG2b isotype control, anti-keyhole limpet hemocyanin(货号BP0090)

推荐抗体稀释液

InVivoPure pH 6.5 Dilution Buffer(货号IP0065)

 


为什么选择InVivoPlus抗体?

InVivoPlus级别的产品内毒素含量更低,经过多种实验验证,更适合用于体内实验研究

BioXcell热销产品推荐--InVivoPlus anti-mouse PD-L1 (B7-H1)


该产品自上市已被多篇SCI文献引用,品质有保证,以下是部分已发表的文献引用:



应用

文章

体内PD-L1阻断

(in vivo PD-L1 blockade)

1. Grasselly, C., et al. (2018). 'The Antitumor Activity of Combinations of Cytotoxic Chemotherapy and Immune Checkpoint Inhibitors Is Model-Dependent' Front Immunol 9: 2100.

2. Stathopoulou, C., et al. (2018). 'PD-1 Inhibitory Receptor Downregulates Asparaginyl Endopeptidase and Maintains Foxp3 Transcription Factor Stability in Induced Regulatory T Cells' Immunity 49(2): 247-263 e247.

3. Kim, J., et al. (2015). 'Memory programming in CD8(+) T-cell differentiation is intrinsic and is not determined by CD4 help' Nat Commun 6: 7994.

体内PD-L1阻断,流式细胞术

(in vivo PD-L1 blockade, Flow Cytometry)

1. Aloulou, M., et al. (2016). 'Follicular regulatory T cells can be specific for the immunizing antigen and derive from naive T cells' Nat Commun 7: 10579.

2. Ngiow, S. F., et al. (2015). 'A Threshold Level of Intratumor CD8+ T-cell PD1 Expression Dictates Therapeutic Response to Anti-PD1' Cancer Res 75(18): 3800-3811.

3. Rutigliano, J. A., et al. (2014). 'Highly pathological influenza A virus infection is associated with augmented expression of PD-1 by functionally compromised virus-specific CD8+ T cells' J Virol 88(3): 1636-1651.

体内PD-L1阻断,免疫荧光

(in vivo PD-L1 blockade, Immunofluorescence)

1.Willimsky, G., et al. (2013). 'Virus-induced hepatocellular carcinomas cause antigen-specific local tolerance' J Clin Invest 123(3): 1032-1043.

免疫组织化学(冷冻切片),免疫荧光

(Immunohistochemistry (frozen), Immunofluorescence)

1.Riella, L. V., et al. (2011). 'Essential role of PDL1 expression on nonhematopoietic donor cells in acquired tolerance to vascularized cardiac allografts' Am J Transplant 11(4): 832-840.



 

BioXcell热销产品推荐--InVivoPlus anti-mouse PD-L1 (B7-H1) 

更多产品详情请联系 BioXcell 中国授权代理——欣博盛生物