荧光淬灭又称荧光熄灭或萃灭,是指导致特定物质的荧光强度和寿命减少的所有现象。引起荧光淬灭的物质称为荧光淬灭剂。SouthernBiotech专门开发的Fluoromount-G系列荧光封片剂是以甘油为基础,加入抗荧光淬灭剂,可明显降低荧光淬灭现象,提高样本中荧光信号的稳定性和持久性。
Fluoromount-G®(货号:0100-01)
Fluoromount-G®是一种水溶性封片剂,用来对最后一步染色为水溶液体系的玻片样本进行封片,可以明显降低荧光显微镜下玻片样本分析中的荧光淬灭现象。据报道,Fluoromount-G®的折射率约为1.4。Fluoromount-G®已有超过20,000多篇文献引用,与100多种荧光染料和试剂兼容。具体详情,请联系SouthernBiotech代理商欣博盛生物!
图1.用抗BCAS2抗体和抗PLZF抗体对小鼠P8睾丸的石蜡切片进行染色。用Hoechst 33342对DNA进行染色,并用Fluoromount-G®(货号0100-01)封片。图片来自Liu W , Wang F , Xu Q, Shi J , Zhang X , Liu X , et al . BCAS2 is involved in alternative mRNA splicing in spermatogonia and the transition to meiosis. Nat Commun. 2017; 8: 14182.
图2. 用AF488标记的鬼笔环肽对卷身罗斯考夫蠕虫胚胎全标本进行染色,并用Fluoromount-G®(货号0100-01)封片。图片来自Semmler H, Bailly X, Wanninger A. Myogenesis in the basal bilaterian Symsagittifera roscoffensis (Acoela). Front Zool.2008; 5:14.
图3. 用anti-Zpr3抗体对冰冻斑马鱼视网膜切片进行染色,然后用AF543二抗和MBODEC染色并用Fluoromount-G®(货号0100-01)封片。图片来自Watanabe K ,Nishimura Y, Oka T, Nomoto T, Kon T, Shitou T,et al. In vivo imaging of zebrafish retinal cells using fluorescent coumarin derivatives. BMC Neurosci.2010; 11:116.
DAPI Fluoromount-G®(货号:0100-20)
DAPI Fluoromount-G®是一种水溶性的、含DAPI的化合物,用来对最后一步染色是在水溶液体系中完成的玻片样本进行封片。当与DNA结合时,DAPI会发出荧光,通常用作细胞核/染色体复染物。由于DAPI Fluoromount-G®是水溶性的,可以通过将玻片浸入PBS溶液中直到盖玻片松开来移除盖玻片。
图4.HCC827细胞先用抗E-钙粘蛋白抗体染色,随后用二抗染色,最后用DAPI Fluoromount-G®(货号0100-20)封片。图片来自Narita M, Shimura E, Nagasawa A , Aiuchi T , Suda Y ,Hamada Y et al . Chronic treatment of non-small-cell lung cancer cells with gefitinib leads to an epigenetic loss of epithelial properties associated with reductions in microRNA-155 and -200c. PLOS One.2017; 12 (2): e0172115.
图5.在无血清培养基中生长的人中性粒细胞用抗中性粒细胞弹性蛋白酶抗体染色,然后用AF555偶联的二抗染色,并用DAPI Fluoromount-G®(货号0100-20)封片。图片来自Kamoshida G, Kikuchi-Ueda T, Nishida S, Tansho-Nagakawa S , Kikuchi H, Ubagai T , et al . Spontaneous formation of neutrophil extracellular traps in serum-free culture conditions. FEBS Open Bio. 2017; 7: 877-86.
Fluoromount-G® Anti-Fade(货号:0100-35)
Fluoromount-G®Anti-Fade是一种非硬化的水基化合物,用于显微镜应用中对荧光强度保持至关重要的载玻片的封片,适用于石蜡切片、冰冻切片及细胞样本。Fluoromount-G® Anti-Fade含有专有的防淬灭成分,可在分析过程中抑制光漂白,非常适合荧光强度至关重要的显微镜应用。
图6.冷冻新生小鼠软骨切片用正常兔血清封闭,用小鼠抗II型胶原蛋白抗体染色,随后用BIOT标记的兔抗小鼠IgG(H+L)抗体(货号:6125-08)、FITC标记的链霉亲和素(货号:7100-02)、DAPI染色,最后用Fluoromount-G® Anti-Fade(货号:0100-35)封片。
图7. 用小鼠抗人CD44-UNLB(货号9400-01)对人胰腺癌细胞系MIA-Paca-2染色,然后用山羊抗小鼠IgG,Human ads-BIOT(货号1030-08)和链霉亲和素-CY3(货号7100-12)染色。
SouthernBiotech的Fluoromount-G系列荧光封片剂具有半永-久封固性,可用作长期保存玻片样本的制作。
产品信息
货号 | 产品名称 | 规格 |
0100-01 | Fluoromount-G® | 25 mL |
0100-20 | DAPI Fluoromount-G® | 20 mL |
0100-35 | Fluoromount-G® Anti-Fade | 20 mL |
详情请联系SouthernBiotech全国授权一级代理——欣博盛生物