Epicypher欣博盛生物—CUTANA™ E. coli Spike-in DNA

更新时间：2023-11-08 点击次数：170次

来源于Escherichia coli（E.coli）的片段DNA可以用作核酸酶靶向切割和释放（CUT&RUN）的实验标准化的spike-in对照。产品CUTANA™ E. coli Spike-in DNA含有足够的材料，可用于100-200个CUT&RUN样本（高丰度和低丰度靶标的范围）。

产品详情

保存温度: Stable for 2 years at -20°C from date of receipt. After resuspending, aliquots should be stored at -20°C.

运输温度: Room temp. Can go on frozen cold packs or dry ice.

产品形式: 100 ng lyophilized DNA. *NOTE: May not be visible.

验证数据

Figure 1: DNA gel data

E. coli fragment size distribution. Lane 1: gDNA extracted from JM101 E.

coli cells (500 ng). Lane 2: Digested and purified CUTANA™ E. coli Spike-in

DNA (500 ng) resolved via 2% E-Gel™ EX Agarose Gel. Migration positions

of DNA molecular weight markers are indicated.

Figure 2: CUT&RUN sequencing data

CUTANA™ E. coli Spike-in DNA (1.0 ng) was added to CUT&RUN samples using 500,000 K562 cells enriched

for a low abundance target (H3K4me3, EpiCypher 13-0041), a high abundance target (H3K27me3, EpiCypher

13-0030) and an IgG negative control (EpiCypher 13-0042). Total numbers of paired-end sequencing reads, reads

aligned to E. coli, and percentage of total sequencing reads aligned to E. coli spike-in DNA are shown. NOTE:

Spike-in DNA amount should be optimized by the end user with the goal of E. coli DNA comprising ~1% (0.2 -

5%) of total sequencing reads.

订购详情

货号	产品名称	规格
18-1401	CUTANA™ E. coli Spike-in DNA	100 ng

Epicypher热销产品——CUTANA™ pAG-Tn5 for CUT&Tag

如需了解更多详细信息或相关产品，请联系EpiCypher中国授权代理商-欣博盛生物

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