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Epicypher热销产品—CUTANA™ E. coli Spike-in DNA

更新时间:2023-12-14   点击次数:203次

来源于Escherichia coli(E.coli)的片段DNA可以用作核酸酶靶向切割和释放(CUT&RUN)的实验标准化的spike-in对照。产品CUTANA™ E. coli Spike-in DNA含有足够的材料,可用于100-200个CUT&RUN样本(高丰度和低丰度靶标的范围)。


产品详情

保存温度: Stable for 2 years at -20°C from date of receipt. After resuspending, aliquots should be stored at -20°C.

运输温度: Room temp. Can go on frozen cold packs or dry ice.

产品形式: 100 ng lyophilized DNA. *NOTE: May not be visible.


如需购买Epicypher产品,或咨询产品技术问题,请联系代理商欣博盛生物


验证数据

Epicypher热销产品——CUTANA™ E. coli Spike-in DNA

Figure 1: DNA gel data

E. coli fragment size distribution. Lane 1: gDNA extracted from JM101 E. 

coli cells (500 ng). Lane 2: Digested and purified CUTANA™ E. coli Spike-in 

DNA (500 ng) resolved via 2% E-Gel™ EX Agarose Gel. Migration positions 

of DNA molecular weight markers are indicated.


Epicypher热销产品——CUTANA™ E. coli Spike-in DNA

Figure 2: CUT&RUN sequencing data

CUTANA™ E. coli Spike-in DNA (1.0 ng) was added to CUT&RUN samples using 500,000 K562 cells enriched 

for a low abundance target (H3K4me3, EpiCypher 13-0041), a high abundance target (H3K27me3, EpiCypher 

13-0030) and an IgG negative control (EpiCypher 13-0042). Total numbers of paired-end sequencing reads, reads 

aligned to E. coli, and percentage of total sequencing reads aligned to E. coli spike-in DNA are shown. NOTE: 

Spike-in DNA amount should be optimized by the end user with the goal of E. coli DNA comprising ~1% (0.2 - 

5%) of total sequencing reads.


订购详情

货号

产品名称

规格

18-1401

CUTANA™ E. coli Spike-in DNA

100 ng



Epicypher热销产品——CUTANA™ pAG-Tn5 for CUT&Tag

如需了解更多详细信息或相关产品,请联系EpiCypher中国授权代理商-欣博盛生物