线粒体膜电位细胞毒性检测试剂盒介绍

Enzo Life Sciences的MITO-ID® Membrane potential cytotoxicity kit利用阳离子双发射染料检测线粒体膜电位（MMP）的波动，该染料在细胞质中以绿色荧光单体的形式存在，在线粒体中以橙色荧光J聚集体的形式聚集。具有低膜电位的线粒体将积累低浓度的染料并呈现绿色荧光，而更高度极化的线粒体将呈现橙红色荧光。随着线粒体功能受损加剧，细胞表现出从橙色荧光到绿色荧光的转变。该试剂盒是一种独-特的HTS测定法，无需洗涤或去除培养基即可实时监测线粒体膜电位。

产品特点

● 灵敏度是JC-1荧光染料的10倍，并具有卓-越的水溶性

● 具备光稳定性的双发射染料

● 无需漂洗/换液步骤

● 单独的MITO-ID®检测可用于检测线粒体质量

● 可在较低的药物/剂量浓度下检测细胞毒性

● 没有使用JC-1染料时出现的溶剂伪影

● 适用于高通量应用

如需购买Enzo Life Sciences产品或咨询技术问题，请联系Enzo Life Sciences代理商欣博盛生物

实验示例

图1. 检测线粒体紊乱的灵敏度是JC-1的10倍。使用MITO-ID®膜电位染料（红色）或JC-1（蓝色）在经CCCP处理的HeLa细胞中评估线粒体膜电位（MMP）。使用传统的荧光酶标仪检测，结果显示MMP 随着CCCP浓度的增加而减少，橙色荧光减少。染料的水溶性优化和无需洗涤的实验方案最大限度减小了可变性，因此Z因子（>0.9）高于使用JC-1.

图2. 在药物筛选中实时检测有丝分裂毒性。使用BioTek Synergy™ Mx荧光酶标仪对线粒体膜电位变化的时间进程研究。HeLa细胞与MITO-ID®膜电位染料在室温下孵育30分钟（不去除血清或培养基）。添加鱼藤酮分别达到1µM、3µM和9µM的浓度。橙色信号的减少证明染料对鱼藤酮有反应。

产品信息

部分产品引用文献

1. Novel Silver Complexes Based on Phosphanes and Ester Derivatives of Bis(pyrazol-1-yl)acetate Ligands Targeting TrxR: New Promising Chemotherapeutic Tools Relevant to SCLC Management: M. Pellei, et al.; Int. J. Mol. Sci. 24, 4091 (2023)

2. FUNDC1 regulates receptor-mediated mitophagy independently of the PINK1/Parkin-dependent pathway in rotenone-treated SH-SY5Y cells: S.Y. Park, et al.; Food Chem. Toxicol. 137, 111163 (2020), Application(s): Fluorescence microscopy and microplate reader

3. Inhibitory role of TRIP-Br1/XIAP in necroptosis under nutrient/serum starvation: Z. Sandag, et al.; Mol. Cells 43, 236 (2020)

4. NAD hydrolysis by the tuberculosis necrotizing toxin induces lethal oxidative stress in macrophages: D. Pajuelo, et al.; Cell. Microbiol. 22, e13115 (2020), Application(s): THP-1 macrophages; microplate reader

5. Impaired autophagic and mitochondrial functions are partially restored by ERT in Gaucher and Fabry diseases: M.M. Ivanova, et al.; PLoS One 14, e0210617 (2019), Application(s): Fluorescence microscopy

6. Inhibitory role of AMPactivated protein kinase in necroptosis of HCT116 colon cancer cells with p53 null mutation under nutrient starvation: D.T. Le, et al.; Int. J. Oncol. 54, 702 (2019)

7. ROS as a novel indicator to predict anticancer drug efficacy: T. Zaidieh, et al.; BMC Cancer 19, 1224 (2019)

8. TREM1/3 deficiency impairs tissue repair after acute kidney injury and mitochondrial metabolic flexibility in tubular epithelial cells: A. Tammaro, et al.; Front. Immunol. 10, 1469 (2019), Application(s): Microplate reader

9. Anti-cancerous effect of cis-khellactone from Angelica amurensis through the induction of three programmed cell deaths: S. Jung, et al.; Oncotarget 9, 16744 (2018), Application(s): Microplate reader

10. Blue light phototoxicity toward human corneal and conjunctival epithelial cells in basal and hyperosmolar conditions: V. Marek, et al.; Free Radic. Biol. Med. 126, 27 (2018), Application(s): Microplate reader

11. cGAS drives noncanonical-inflammasome activation in age-related macular degeneration: N. Kerur, et al.; Nat. Med. 24, 50 (2018)

12. Cytotoxicity of propofol in human induced pluripotent stem cell-derived cardiomyocytes: K. Kido, et al.; J. Anesth. 32, 120 (2018), Application(s): Microplate reader

13. Development of novel amino-quinoline-5, 8-dione derivatives as NAD (P) H: quinone oxidoreductase 1 (NQO1) inhibitors with potent antiproliferative activities: Y. Ling, et al.; Eur. J. Med. Chem. 154, 199 (2018)

14. Inhibition of apoptosis using exosomes in Chinese hamster ovary cell culture: S. Han, et al.; Biotechnol. Bioeng. 115, 1331 (2018)

15. Light action spectrum on oxidative stress and mitochondrial damage in A2E-loaded retinal pigment epithelium cells: M. Marie, et al.; Cell Death Disc. 9, 287 (2018)

详情请联系Enzo Life Sciences金牌代理——欣博盛生物