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Epicypher:Mononucleosomes, Recombinant Human

更新时间:2023-12-21   点击次数:128次

重组人单核小体,由大肠杆菌中表达的重组人组蛋白组装而成(组蛋白H2AH2BH3H42;Accession numbers:H2A-P04908;H2B-O60814;H3.1-P68431;H4- P62805),缠绕着147个碱基对包含601序列DNA核小体是染色质的基本亚基。由LowaryWidom鉴定的601序列对组蛋白八聚体具有高亲和力,有利于核小体的组装。


产品详情

宿主来源: Human

表达系统: E. coli & synthetic DNA

保存温度: Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid freeze/thaws

运输温度: Dry ice

产品形式: 0.84 mg/mL mononucleosome in µL 10 mM Tris-HCl pH 7.5, 25 mM NaCl, 1 mM EDTA, 2 mM DTT, and 20% glycerol. (54.6 µg protein, 100 µg DNA + protein)


Epicypher热销产品——Mononucleosomes, Recombinant Human

Figure 1: DNA Gel Data

Mononucleosomes, Recombinant Human run on an agarose gel and stained with ethidium bromide to visualize DNA. Lane 1: Free DNA extracted from nucleosomes (200 ng). Lane 2: Intact nucleosomes (400 ng).


Epicypher热销产品——Mononucleosomes, Recombinant Human

Figure 2: Protein Gel Data

Coomassie stained PAGE gel of proteins in Mononucleosomes, Human Recombinant (0.5 µg) to demonstrate the purity and integrity of the histones in the preparation. Sizes of molecular weight markers and positions of the core histones (H2A, H2B, H3 and H4) are indicated.

Epicypher热销产品——Mononucleosomes, Recombinant Human

Figure 3: HMTase Assay Data

Mononucleosomes, Recombinant Human used as a substrate in a radioactive histone methyltransferase assay with (+SET8) or without (-SET8) recombinant SET8, which is an HMTase that requires nucleosomal substrates for activity.


产品使用文献

[1] Huang, et al. Oncohistone Mutations Occur at Functional Sites of Regulatory ADP-ribosylation. Cancer Res, 2022.

[2] Jabeena, et al. Dynamic association of the H3K64 trimethylation mark with genes encoding exported proteins in Plasmodium falciparum. JBC, 2021.

[3] Yuan, et al. Elevated NSD3 histone methylation activity drives squamous cell lung cancer. Nature, 2021.

[4] Mullahoo, et al. Dual protease type XIII/pepsin digestion offers superior resolution and overlap for the analysis of histone tails by HX-MS. Methods, 2020

[5] Yan, et al. Deficient histone H3 propionylation by BRPF1-KAT6 complexes in neurodevelopmental disorders and cancer.Science Advances, 2020.


订购详情

货号

产品名称

规格

16-0006

Mononucleosomes, Recombinant Human Biotinylated

50 µg



Epicypher热销产品——CUTANA™ pAG-Tn5 for CUT&Tag

如需了解更多详细信息或相关产品,请联系EpiCypher中国授权代理商-欣博盛生物