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ROS-ID®一氧化氮检测试剂盒:ENZO热销产品

更新时间:2024-02-04   点击次数:56次

Enzo Life SciencesROS-ID® NO Detection kit红色荧光的NO检测试剂为主要成分,通过荧光显微镜直接实时监测活细胞中NO的产生。这种非荧光的、可渗透细胞的NO检测染料在O2存在的情况下能够与NO发生反应,具有高特异性、灵敏性和准确性,产生不溶于水的红色荧光产物。这种染料对过氧亚硝酸盐没有反应性,因此可以区分过氧亚硝酸盐和一氧化氮。

ROS-ID® NO Detection kit中的荧光探针对活性氯或溴不敏感,因此不用于检测这些分析物。染色后,该染料产生的荧光产物可通过荧光显微镜进行观察。当需要同时检测额外的荧光信号(绿色或橙色)时,建议使用650/670 nm的组合。ROS-ID® NO Detection kit包含足够的试剂,可对活细胞(贴壁或悬浮)进行至少200次检测。

 

产品特色

通过荧光显微镜直接实时监测活体细胞中NO的产生

高特异性、灵敏性和准确性,产生不溶于水的红色荧光产物

可区分过氧亚硝酸盐和一氧化氮

试剂盒内包含一氧化氮诱导剂和清除剂

 

产品信息

产品货号

ENZ-51013-200

产品名称

ROS-ID® NO Detection kit / 欣博盛生物

规格

1*1Kit

操作

Protect from light. Avoid freeze/thaw cycles.

短期保存

-20°C

长期保存

-80°C

试剂盒组分

NO Detection Reagent (Red), 60 µl

NO Inducer (L-Arginine), 100 µl

NO Scavenger (c-PTIO), 400 nmoles

10X Wash Buffer, 15 ml

应用

Fluorescence microscopy, Fluorescent detection

 

部分产品引用文献

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2. Noncanonical Role of Telomerase in Regulation of Microvascular Redox Environment With Implications for Coronary Artery Disease: K.A. Aissa, et al.; Function 3, zqac043 (2022)

3. S1P (Sphingosine-1-Phosphate)-Induced Vasodilation in Human Resistance Arterioles During Health and Disease: B. Katunaric, et al.; Hypertension 79, 2250 (2022)

4. High-content analysis monitoring intracellular trafficking and replication of Mycobacterium tuberculosis inside host cells: N. Debousere, et al.; Methods Mol. Biol. 2314, 649 (2021)

5. Hyperhomocysteinemia and Low Folate and Vitamin B12 Are Associated with Vascular Dysfunction and Impaired Nitric Oxide Sensitivity in Morbidly Obese Patients: M. Haloul, et al.; Nutrients 12, 2014 (2020)

6. Stimulation of TRPA1 attenuates ischemia-induced cardiomyocyte cell death through an eNOS-mediated mechanism: S.R. Andrei, et al.; Channels (Austin) 13, 192 (2019)

7. Anti-inflammatory effects of olive-derived hydroxytyrosol on lipopolysaccharide-induced inflammation in RAW264.7 cells: Y. Yonezawa, et al.; J. Vet. Med. Sci. 80, 1801 (2018)

8. Oligodendrocyte RasG12V expressed in its endogenous locus disrupts myelin structure through increased MAPK, nitric oxide, and notch signaling: H.E. Titus, et al.; Glia 65, 1990 (2017)

9. Improved arterial flow-mediated dilation after exertion involves hydrogen peroxide in overweight and obese adults following aerobic exercise training: A.T. Robinson, et al.; J. Hypertens. 34, 1309 (2016)

10. STAT3 Represses Nitric Oxide Synthesis in Human Macrophages upon Mycobacterium tuberculosis Infection: C.J. Queval, et al.; Sci. Rep. 6, 29297 (2016)

11. Flavonoids activate endothelial nitric oxide synthase by altering their phosphorylation via mitogen-activated protein kinase pathways in glucose-induced endothelial cells: C.E. Kim, et al.; J. Funct. Foods 17, 676 (2015)

12. Ginsenoside Rg3 regulates S-Nitrosylation of the NLRP3 inflammasome via suppression of iNOS: S. J. Yoon, et al.; Biochem. Biophys. Res. Commun. 463, 1184 (2015)

13. Propofol restores TRPV1 sensitivity via a TRPA1-, nitric oxide synthase-dependent activation of PKCε: P. Sinharoy, et al.; Pharmacol. Res. Perspect. 3, e00153 (2015)

14. Reduced flow-and acetylcholine-induced dilations in visceral compared to subcutaneous adipose arterioles in human morbid obesity: I. Grizelj, et al.; Microcirculation 22, 44 (2015)

15. Anti-inflammatory effects of Edaravone and Scutellarin in activated microglia in experimentally induced ischemia injury in rats and in BV-2 microglia: Y. Yuan, et al.; BMC Neurosci. 15, 125 (2014)

16. Lipopolysaccharide (LPS) stimulation of fungal secondary metabolism: Z.G. Khalil, et al.; Mycology 5, 168 (2014)

17. Cyclosporine attenuates arginine transport, in human endothelial cells, through modulation of cationic amino acid transporter-1: A. Grupper, et al.; Am. J. Nephrol. 37, 613 (2013)

18. Identification and characterization of a functional mitochondrial angiotensin system: P.M. Abadir, et al.; PNAS 108, 14849 (2011)

19. Loss of bcl-2 during the senescence exacerbates the impaired angiogenic functions in endothelial cells by deteriorating the mitochondrial redox state: M. Uraoka, et al.; Hypertension 58, 254 (2011)

 

 


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