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Epicypher:CTCF CUTANA™ CUT&RUN Antibody

更新时间:2024-02-05   点击次数:60次

EpiCypher是一家为表观遗传学和染色质生物学研究提供高质量试剂和工具的专业制造商。EpiCypher推出的CUT&RUN级别的CTCF Antibody符合EpiCypher的“CUTANA Compatible"标准,可用于核酸酶靶向切割和释放(CUT&RUN)和/或靶向剪切及转座酶技术(CUT&Tag)的基因组定位。CTCF通过结合DNA并阻止启动子和附近增强子或沉默子之间的相互作用来调节转录。


产品详情

反应种属: Human, Mouse

宿主来源: Rabbit

实验应用: CUT&RUN, IHC, ICC, IP, WB

免疫原: Between amino acids 650 and 700

克隆性: Monoclonal [BLR041F]

表达系统: HEK293 cells

保存温度: Stable for 1 year at 4°C from date of receipt

运输温度: DO NOT FREEZE!! Frozen cold packs.

产品形式: Purified recombinant monoclonal antibody in borate buffered saline pH 8.2, 0.1% BSA, 0.09% sodium azide


数据示例

Epicypher热销产品——CTCF CUTANA™ CUT&RUN Antibody

Figure 1: CTCF peaks in CUT&RUN

CUT&RUN was performed as described above. Peaks were called with MACS2. Heatmaps show CTCF peaks relative to IgG negative control antibody in aligned rows ranked by intensity (top to bottom) and colored such that red indicates high localized enrichment and blue denotes background signal. CTCF showed expected enrichment around the TSS. Despite some observable background in the IgG control, differential enrichment with the target antibody is as expected.

Epicypher热销产品——CTCF CUTANA™ CUT&RUN Antibody 

Figure 2: CTCF CUT&RUN representative browser tracks

CUT&RUN was performed as described above. Gene browser shots were generated using the Integrative Genomics Viewer (IGV, Broad Institute). Three representative loci are shown.

Epicypher热销产品——CTCF CUTANA™ CUT&RUN Antibody

Figure 3: Immunohistochemistry data

FFPE sections of human colon carcinoma (left) and mouse spleen (right) using CTCF antibody at a dilution of 1:1000.

Epicypher热销产品——CTCF CUTANA™ CUT&RUN Antibody

Figure 4: Immunocytochemistry data

FFPE section of human MCF-7 cells examined using CTCF antibody at a dilution of 1:1,000.

Epicypher热销产品——CTCF CUTANA™ CUT&RUN Antibody

Figure 5: Immunoprecipitation data

EpiCypher CTCF antibody (6 μg/mg lysate) was used to immunoprecipitate whole cell lysates (1 mg, 20% of IP loaded) isolated from HEK293T cells. A negative control IgG antibody and positive control antibodies (Bethyl Laboratories) were also used to demonstrate specificity of the IP. All CTCF antibodies target the same CTCF epitope, however, A300-534A-6 is hosted in rabbit. For blotting immunoprecipitates, EpiCypher CTCF antibody was used at a 1:1,000 dilution.

Epicypher热销产品——CTCF CUTANA™ CUT&RUN Antibody

Figure 6: Western blot data

Western analysis of CTCF in whole cell extracts from HEK293T, MCF-7, Jurkat, Hep-G2, and A-549 cells. Fifty micrograms of lysate was resolved via SDS-PAGE and detected with a 1:1,000 dilution of CTCF antibody.


订购详情

货号

产品名称

规格

13-2014

CTCF CUTANA™ CUT&RUN Antibody

100 µL

 

 

Epicypher热销产品——CUTANA™ pAG-Tn5 for CUT&Tag

如需了解更多详细信息或相关产品,请联系EpiCypher中国授权代理商-欣博盛生物