MEGACD40L®是一种高活性蛋白,其中的两个三聚体CD40配体分子通过Adiponectin(脂联素)/ACRP30/AdipoQ的胶原结构域人工连接。该蛋白质非常有效地模拟了在体内自然条件下,CD40L的膜辅助聚集,为[CD40L+增强剂]的组合(产品货号:ALX-850-064)提供了一种简单而同样有效的替代方案。
产品信息
产品名称:MEGACD40L® Protein (soluble) (human), (recombinant) /欣博盛生物
别名:CD40L:ACRP30headless, CD154:ACRP30headless, TNFSF 5:ACRP30headless, gp39:ACRP30headless
来源:CHO cells
*The extracellular domain of human CD40L (CD154) (aa 116-261) is fused at the N-terminus to mouse ACRP30headless (aa 18-111) and a FLAG®-tag.
UniProt ID: P29965 (human CD40L), Q60994 (mouse Adiponectin)
浓度:0.1mg/ml after reconstitution
纯度:≥90% (SDS-PAGE)
内毒素含量:≤0.01EU/µg purified protein (LAL test; Cape Cod Associates)
生物活性:Binds to human CD40. Induces B cells activation (as demonstrated by dose-dependent upregulation of CD86).
产品应用
CD40L用于构建共刺激激活免疫反应(特别是B细胞)。可作为免疫治疗临床前研究的蛋白质工具,用于介导免疫和炎症反应,监测接受化疗的患者,并在体内模拟自身免疫表型。
应用关键词:自身免疫性疾病,B细胞活化,CD40,CD40L,共刺激,免疫刺激,免疫治疗,疫苗开发
产品特点
配方经过优化,在提高产品稳定性的同时,还增强了产品活性;
无需额外的增强剂,效果优于单独CD40L(货号ALX-522-015)或CD40L+增强剂组合(货号:ALX-850-064);
高质量的免疫刺激,具有强效和持续的效果;
高纯度,内毒素含量低(≤0.01 EU/μg);
批次间差异小,具有高稳定性和高一致性;
ENZO最-畅-销的蛋白质之一,国际知-名度高,已发表文献引用高达58次。
产品结构
货号:ALX-522-110
MEGACD40L® Protein (soluble) (human), (recombinant)结构示意图
品牌 | 来源 | 序列 | 内毒素含量 | 状态 | 生物活性 | 引用文献 |
ENZO | CHO cells | Human CD40L (CD154) (aa 116-261) is fused at the N-terminus to mouse ACRP30headless (aa 18-111) and a FLAG® tag | ≤ 0.01 EU/µg purified protein (LAL test; Cape Cod Associates) | Lyophilized. Contains PBS | Binds to human CD40. Induces B cell activation (as demonstrated by dose-dependant upregulation of CD86) | 58 |
某知-名-品-牌 | < 0.01 EU/µg purified protein (LAL test; Lonza) | 13 |
与知-名-品-牌相比文献引用数量领-先
订购详情
产品名称 | 货号 | 规格 |
MEGACD40L® Protein (soluble) (human), (recombinant) | ALX-522-110 | 10 µg |
最新引用文献
Distinct stage-specific transcriptional states of B cells derived from human tonsillar tissue: D.A. Espinoza, et al.; JCI Insight 8, e155199 (2023), Abstract;
PD-1highCXCR5–CD4+ peripheral helper T cells promote CXCR3+ plasmablasts in human acute viral infection: H. Asashima, et al.; Cell Rep. 42, 111895 (2023), Abstract;
An optimized measles virus glycoprotein-pseudotyped lentiviral vector production system to promote efficient transduction of human primary B cells: E. Vamva, et al.; STAR Protoc. 3, 101228 (2022), Abstract;
详情请联系Enzo Life Sciences金牌代理——欣博盛生物