FGK4.5-CP133单克隆抗体是原始FGK4.5单克隆抗体的重组嵌合型抗体。可变结构域序列与原始FGK4.5克隆号相同,但是恒定区序列已经从大鼠IgG2a变为小鼠IgG2a。FGK4.5-CP133抗体像原始大鼠IgG2a抗体一样,不包含Fc突变。
FGK4.5-CP133单克隆抗体能与小鼠CD40(也称为Bp50)反应。CD40是一种48 kDa的I型跨膜糖蛋白,属于肿瘤坏死因子受体(TNFR)超家族。CD40广泛表达于抗原呈递细胞(APCs),如树突状细胞、B细胞、巨噬细胞和单核细胞,以及非免疫内皮细胞、基底上皮细胞和一系列肿瘤细胞上。CD40与其配体CD154结合后,作为共刺激分子激活B细胞、树突状细胞、单核细胞和其他APCs。CD40在B细胞活化、分化、增殖和免疫球蛋白同种型转换以及树突状细胞成熟中起作用。激动性CD40单克隆抗体已被证明能激活APCs并促进抗肿瘤T细胞反应。FGK4.5单克隆抗体是一种激动性抗体,已被证明能激活表达CD40的APC。FGK4.5单克隆抗体也可用于体外和体内抑制CD40/CD154相互作用。
产品详情:
产品名称 | RecombiMAb anti-mouse CD40 /欣博盛生物 |
产品货号 | CP133 |
产品规格 | 1/5/25/50/100mg |
反应种属 | Mouse |
克隆号 | FGK4.5-CP133 |
同种型 | Mouse IgG2a(switched from rat IgG2a) |
免疫原 | Recombinant mouse CD40 fusion protein |
实验应用 | in vivo CD40 activation* |
产品形式 | PBS, pH 7.0,Contains no stabilizers or preservatives |
纯度 | >95%, Determined by SDS-PAGE |
聚合 | <5%, Determined by SEC |
无菌处理 | 0.2 µm filtration |
纯化方式 | Protein G |
分子量 | 150 kDa |
小鼠病原检测 | Ectromelia/Mousepox Virus: Negative Hantavirus: Negative K Virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Lymphocytic Choriomeningitis virus: Negative Mouse Adenovirus: Negative Mouse Cytomegalovirus: Negative Mouse Hepatitis Virus: Negative Mouse Minute Virus: Negative Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Rotavirus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Polyoma Virus: Negative Reovirus Screen: Negative Sendai Virus: Negative Theiler’s Murine Encephalomyelitis: Negative |
保存条件 | 抗体原液保存在4°C,不能冷冻保存。 |
推荐同型对照 | InVivoPlus mouse IgG2a isotype control, unknown specificity(货号BP0085) |
推荐抗体稀释液 | InVivoPure pH 7.0 Dilution Buffer(货号IP0070) |
该产品自上市已被多篇SCI文献引用,品质有保证,以下是部分已发表的文献引用:
应用 | 文章 |
体内CD40激活 (in vivo CD40 activation) | 1. Bauche, D., et al. (2018). 'LAG3(+) Regulatory T Cells Restrain Interleukin-23- Producing CX3CR1(+) Gut-Resident Macrophages during Group 3 Innate Lymphoid Cell-Driven Colitis' Immunity 49(2): 342-352 e345. 2. Carmi, Y., et al. (2015). 'Allogeneic IgG combined with dendritic cell stimuli induce antitumour T-cell immunity' Nature 521(7550): 99-104. 3. Conde, P., et al. (2015). 'DC-SIGN(+) Macrophages Control the Induction of Transplantation Tolerance' Immunity 42(6): 1143-1158. 4. Bartkowiak, T., et al. (2015). 'Unique potential of 4-1BB agonist antibody to promote durable regression of HPV+ tumors when combined with an E6/E7 peptide vaccine' Proc Natl Acad Sci U S A 112(38): E5290-5299. |
体外B细胞刺激/激活 (in vitro B cell stimulation/activation) | 1. Xu, H., et al. (2015). 'Regulation of bifurcating B cell trajectories by mutual antagonism between transcription factors IRF4 and IRF8' Nat Immunol . 2. Muppidi, J. R., et al. (2014). 'Loss of signalling via Galpha13 in germinal centre B-cell-derived lymphoma' Nature 516(7530): 254-258. |
更多产品详情请联系 BioXCell 中国授权代理——欣博盛生物