Alpha Synuclein Oligomer ELISA Kit精准检测α-突触核蛋白寡聚体

本试剂盒采用夹心ELISA技术，可特异性识别人和小鼠 α-突触核蛋白寡聚体，与α-突触核蛋白单体和原纤维的交叉反应性极低(参见图1结果)，确保检测结果真实反映寡聚体水平，有效避免非特异性结合导致的数据偏差。

图1. The graph demonstrates robust detection of Human Recombinant Alpha Synuclein Oligomers (Cat#SPR-484) and limited cross-reactivity with Human Recombinant Alpha Synuclein Pre-Formed Fibrils and Monomers (Cat #s SPR-322 & SPR-321).

试剂盒已针对脑组织匀浆样本进行了全面验证，并在说明书中提供了详细的样品制备方案，帮助您高效处理脑组织样本。在转基因动物模型研究中，可对脑组织匀浆中α-突触核蛋白寡聚体实现稳定、可靠的定量分析(参见图2结果)。

图2. Representative quantification of mouse brain homogenate samples of unknown concentrations of Alpha Synuclein Oligomers against a standard curve of Human Recombinant Alpha Synuclein Oligomers when using StressMarq’s Alpha Synuclein Oligomer ELISA kit (Cat # SKT-143). A: Non-detergent protein extraction from brain homogenates of hemizygous M83 mice injected in the striatum with either Human Recombinant Alpha Synuclein Pre-Formed Fibrils (PFF; n = 3) or with buffer (PBS; n = 3). B: Non-detergent protein extraction from brain homogenates of either homozygous M83 mice (M83 Transgenic; n = 2) or wildtype mice (Wildtype;n = 2). C-D: Detergent-based protein extraction (25 ug/mL) from brain homogenates of hemizygous M83 mice injected in the striatum with either PFF or PBS. Data from males (C: PFF n = 6; PBS n = 6) and females (D: PFF n = 6; PBS n = 5) are presented separately. Experimental data courtesy of Rodrigo Sandoval Contreras and Dareen Abdel-Rahman from the Prado Lab at University of Western Ontario, contributed as part of a collaborative research project.

试剂盒检测灵敏度达10pg/mL，检测范围为15.6-1000pg/mL，可覆盖生理到病理状态下的寡聚体浓度变化。标准曲线线性良好(参见图3结果)，为精确定量提供可靠保障。

试剂盒包含所有必需组分，并配套详细操作说明书。从样品孵育到显色读数，整个流程仅需约3小时，支持高通量检测，可同时检测40个样本(含复孔)。