热门抗体ADAM10 Antibody由ProSci LLC公司研发生产,产品货号2051,有0.02 mg和0.1 mg两种规格可供选择。欣博盛生物是ProSci LLC公司在国内的授权代理商,负责ProSci LLC公司产品在国内销售,以及ProSci LLC公司产品售前技术支持。
产品信息
产品货号 | 2051 |
产品名称 | ADAM10 Antibody |
规格 | 0.02 mg / 0.1 mg |
宿主种属 | 兔 |
克隆性 | Polyclonal |
反应种属 | 人,小鼠,大鼠 |
验证应用 | ELISA, FC, IHC-P, WB, ICC/IF, IHC-P/IF |
偶联 | Unconjugated |
实验图例

图1 ADAM10 KO Validation in MEF Cells
Loading: 10 ug of lysate Antibodies: ADAM10 2051, 1 ug/mL and beta-actin 3779-1301, 1ug/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.2051 detected both precursor ADAM10 (94KD) and mature ADAM10 (68kD).

图2 Immunohistochemistry Validation of ADAM10 in Human Testis
Immunohistochemical analysis of paraffin-embedded human testis tissue using anti-ADAM10 antibody (2051) at 2 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

图3 Immunofluorescence Validation of ADAM10 in Rat Thymus
Immunofluorescent analysis of 4% paraformaldehyde-fixed rat thymus labeling ADAM10 with 2051 at 10 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).
详情请联系ProSci中国代理商——欣博盛生物