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BIOMOL® Green

更新时间:2022-01-28

简要描述:

BIOMOL® Green provides a simple and convenient method for colorimetric phosphate quantitation (abs. 600-680 nm). Unlike other molybdate/malachite green-based assays, BIOMOL® Green doesn’t require ……

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  • Convenient one-step reagent, no mixing needed

  • Extremely stable with a long shelf-life, >6 months at +4°C

  • Excellent sensitivity, ~50 pmol in 100 µl

  • Can be used in cuvette or microplate-based assays, making it ideal for high-throughput applications

  • Simple and complete kit that comes with a standardized phosphate solution for assay calibration

BIOMOL® Green provides a simple and convenient method for colorimetric phosphate quantitation (abs. 600-680 nm). Unlike other molybdate/malachite green-based assays, BIOMOL® Green doesn’t require multiple solutions or reagents prepared fresh on the day of the assay. BIOMOL® Green is extremely stable (>6 mos. at 4°C) and is simply mixed, at room temperature, with any enzymatic reaction that has released free phosphate. Reported applications include assays for phospholipid phosphatases, tyrosyl-tRNA synthetase (coupled with pyrophosphatase) and a viral RNA triphosphatase.BIOMOL® Green has perhaps been most widely applied to protein phosphatase assays. Typically, a protein phosphatase (e.g. PTP1B or LAR) is simply incubated with an appropriate phosphopeptide (e.g. IR5,9,10). Addition of BIOMOL® Green stops the reaction and begins color development, which is read 20-30 min. later. BIOMOL® Green may be used in cuvette or microplate-based assays and is ideal for high-throughput applications. Each kit comes with a standardized phosphate solution for assay calibration.

Product Details


Applications:FUNC, Colorimetric detection, HTS

Application Notes:Mainly used in protein phosphatase assays. Reported applications include assays for phospholipid phosphatases, tyrosyl-tRNA synthetase (coupled with pyrophosphatase) and a viral RNA triphosphatase.

Shipping:Ambient

Long Term Storage:+4°C

Contents:Biomol® Green Reagent (Prod. No. BML-AK111) (1l or 250ml; liquid in screw cap plastic bottle) Storage: room temperature. Long-term at 4°C; can be stored frozen without deleterious effects.
Phosphate Standard (Prod. No. BML-KI102) (1ml; 800µm phosphate in distilled water) Storage: room temperature. Long-term at 4°C; can be stored frozen without deleterious effects.

Regulatory Status:RUO - Research Use Only



Product Literature References


A covalent p97/VCP ATPase inhibitor can overcome resistance to CB-5083 and NMS-873 in colorectal cancer cells: G. Zhang, et al.; Eur. J. Med. Chem. 213, 113148 (2021), Abstract;

A covalent p97/VCP ATPase inhibitor can overcome resistance to CB-5083 and NMS-873 in colorectal cancer cells: G. Zhang, et al.; Eur. J. Med. Chem. 213, 113148 (2021), Abstract;

Biochemical characterization of a recombinant acid phosphatase from Acinetobacter baumannii: E. Smiley- Moreno, et al.; PLoS One 16, e0252377 (2021), Abstract; Full Text

Discovery of a New, Recurrent Enzyme in Bacterial Phosphonate Degradation: ( R)-1-Hydroxy-2-aminoethylphosphonate Ammonia-lyase: E. Zangelmi, et al.; Biochemistry (2021), Abstract;

Discovery of novel antituberculosis agents among 3-phenyl-5-(1-phenyl-1H-[1,2,3]triazol-4-yl)-[1,2,4]oxadiazole derivatives targeting aminoacyl-tRNA synthetases: M. Y. Rybak, et al.; Sci. Rep. 11, 7162 (2021), Abstract; Full Text

Fragment-Based Design of a Potent MAT2a Inhibitor and in Vivo Evaluation in an MTAP Null Xenograft Model: C.D. Fusco, et al.; J. Med. Chem. 1021, 67 (2021), Abstract;

Identification of novel OCRL isoforms associated with phenotypic differences between Dent disease-2 and Lowe syndrome: N. Sakakibara, et al.; Nephrol. Dial. Transplant. (2021), Abstract;

Protein phosphatase 2A holoenzymes regulate leucine-rich repeat kinase 2 phosphorylation and accumulation: M. Drouyer, et al.; Neurobiol. Dis. 157, 105426 (2021), Abstract;

Repurposing p97 inhibitors for chemical modulation of the bacterial ClpB–DnaK bichaperone system: P. Glaza, et al.; J. Biol. Chem. 296, 100405 (2021), Abstract; Full Text

Structural basis for the dynamics of human methionyl-tRNA synthetase in multi-tRNA synthetase complexes: D.K. Kim, et al.; Nucleic Acid Res. 49, 6549 (2021), Abstract; Full Text

The protein phosphatase PPM1A dephosphorylates and activates YAP to govern mammalian intestinal and liver regeneration: R. Zhou, et al.; PLoS Biol. 19, e3001122 (2021), Abstract;

Viral RNA recognition by LGP2 and MDA5, and activation of signaling through step-by-step conformational changes: I. Duic, et al.; Nucleic Acids Res. 48, 11664 (2020), Abstract; Full Text

Crystal structure and catalytic activity of the PPM1K N94K mutant: M. Rostaminasab Dolatabad, et al.; J. Neurochem. 148, 550 (2019), Abstract;

De Novo Mutations Affecting the Catalytic Cα Subunit of PP2A, PPP2CA, Cause Syndromic Intellectual Disability Resembling Other PP2A-Related Neurodevelopmental Disorders: S. Reynhout, et al.; Am. J. Hum. Genet. 104, 139 (2019), Abstract; Full Text

Evaluation of binding and inhibition mechanism of dietary phytochemicals with sphingosine kinase 1: Towards targeted anticancer therapy: P. Gupta, et al.; Sci. Rep. 9, 18727 (2019), Abstract; Full Text

Investigation of inhibitory potential of quercetin to the pyruvate dehydrogenase kinase 3: Towards implications in anticancer therapy: R. Dahiya, et al.; Int. J. Biol. Macromol. 136, 1076 (2019), Abstract;

Lipoxin A4 ameliorates lipopolysaccharide-induced lung injury through stimulating epithelial proliferation, reducing epithelial cell apoptosis and inhibits epithelial–mesenchymal transition: J. Yang, et al.; Respir. Res. 20, 192 (2019), Abstract; Full Text

Probing the Inhibition of Microtubule Affinity Regulating Kinase 4 by N-Substituted Acridones: M. Voura, et al.; Sci. Rep. 9, 1676 (2019), Application(s): Enzyme Activity of MARK4 (Kinase inhibition assay), Abstract; Full Text

Trypanosoma cruzi Phosphomannomutase and Guanosine Diphosphate-Mannose Pyrophosphorylase Ligandability Assessment: F. Zmuda, et al.; Antimicrob. Agents Chemother. 63, e01082-19 (2019), Abstract; Full Text

A dynamic allosteric pathway underlies Rad50 ABC ATPase function in DNA repair: Z.K. Boswell, et al.; Sci Rep. 8, 1639 (2018), Abstract; Full Text

An essential Staphylococcus aureus cell division protein directly regulates FtsZ dynamics: P. Eswara, et al.; Elife 7, 38856 (2018), Abstract; Full Text


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