产品中心您现在的位置:首页 > 产品展示 > > 蛋白&肽 > Guanylyl cyclase α1β1 (soluble) (human), (recombin

Guanylyl cyclase α1β1 (soluble) (human), (recombin

更新时间:2022-01-28

简要描述:

Guanylyl cyclase α1β1 (soluble) (human), (recombinant) (His-tag) (purified)
品牌: ENZO 产品货号: ALX-201-177-C010 规格: 1*10µg
更多ENZO公司的产品,请咨询一级代理商欣博盛生物

型号:厂商性质:代理商浏览量:809

Guanylyl cyclase α1β1 (soluble) (human), (recombinant) (His-tag) (purified)

Product Details

Alternative Name:GTP pyrophosphate-lyase (cycling), sGCα1/sGCβ1

MW:~70kDa/~86kDa / subunit, respectively; heterodimer.

Source:Produced in Sf9 insect cells. Fused at the C-terminus to a His-tag. Produced in a baculovirus expression system.

EC:4.6.1.2

UniProt ID:Q02108 (GCα1), Q02153 (GCβ1)

Formulation:Liquid. In 50 mM Triethanolamine, pH 7.4, containing 150 mM NaCl, and 25% glycerol.

Purity:≥90% (SDS-PAGE)

Specific Activity:Basal: ≥0.050 µmol/mg/min.
- 100µM DEA NONOate (Prod. No. ALX-430-034) ≥10 µmol/mg/min.

Application Notes:Biophysical and enzymatic studies and drug screening.

Shipping:Shipped on Dry Ice

Long Term Storage:-80°C

Use/Stability:We do not recommend to store for longer periods after opening and thawing of the product.

Handling:Avoid freeze/thaw cycles.

Scientific Background:Guanylyl cyclase is part of the G protein signaling cascade that is activated by low intracellular calcium levels and inhibited by high intracellular calcium levels. In response to calcium levels, guanylyl cyclase synthesizes cGMP from GTP. cGMP keeps cGMP-gated channels open, allowing for the entry of calcium into the cell.

Regulatory Status:RUO - Research Use Only

Guanylyl cyclase α1β1 (soluble) (human), (recombinant) (His-tag) (purified) image
Figure: Spectral characteristics of basal and NO-treated purified human sGCα1β1 (3µM). Inset – Coomassie staining of 3µg of purified sGCα1β1. PM- protein marker.
Please mouse over
Guanylyl cyclase α1β1 (soluble) (human), (recombinant) (His-tag) (purified) image


Product Literature References

A new small molecule inhibitor of soluble guanylate cyclase: F. Mota, et al.; Bioorg. Med. Chem. 23, 5303 (2015), Abstract; Full Text
Surface plasmon resonance using the catalytic domain of soluble guanylate cyclase allows the detection of enzyme activators: F. Mota, et al.; Bioorg. Med. Chem. Lett. 24, 1075 (2014), Abstract; Full Text
Abacavir increases platelet reactivity via competitive inhibition of soluble guanylyl cyclase: P.D. Baum, et al.; AIDS 25, 2243 (2011), Application(s): Assay, Abstract; Full Text
Inhibition of nitric oxide-activated guanylyl cyclase by calmodulin antagonists: L.R. James, et al.; Br. J. Pharmacol. 158, 1454 (2009), Abstract; Full Text
Nitric oxide production from nitrite occurs primarily in tissues not in the blood: critical role of xanthine oxidase and aldehyde oxidase: H. Li, et al.; J. Biol. Chem. 283, 17855 (2008), Abstract; Full Text
Human soluble guanylate cyclase: functional expression, purification and structural characterization: D.N. Kosarikov, et al.; Arch. Biochem. Biophys. 388, 185 (2001), Abstract;
Human recombinant soluble guanylyl cyclase: expression, purification, and regulation: Y.C. Lee, et al.; PNAS 97, 10763 (2000), Abstract; Full Text
Purified soluble guanylyl cyclase expressed in a baculovirus/Sf9 system: stimulation by YC-1, nitric oxide, and carbon monoxide: M. Hoenicka, et al.; J. Mol. Med. 77, 14 (1999), Abstract;
Human soluble guanylate cyclase: functional expression and revised isoenzyme family: U. Zabel, et al.; Biochem. J. 335 (Pt 1), 51 (1998), Abstract; Full Text
Soluble guanylate cyclase purified from bovine lung contains heme and copper: R. Gerzer, et al.; FEBS Lett. 132, 71 (1981), Abstract;


如需购买或咨询该产品技术问题,请联系ENZO一级代理商-欣博盛生物

留言框

  • 产品:

  • 您的单位:

  • 您的姓名:

  • 联系电话:

  • 常用邮箱:

  • 省份:

  • 详细地址:

  • 补充说明:

  • 验证码:

    请输入计算结果(填写阿拉伯数字),如:三加四=7