更新时间:2022-03-03
CleanCap Reagent AU is designed for the co-transcriptional capping of mRNA to produce an mRNA with naturally occurring Cap 1. CleanCap Reagent AU was specifically designed for self-replicating RNAs b…
Description
CleanCap Reagent AU is designed for the co-transcriptional capping of mRNA to produce an mRNA with naturally occurring Cap 1. CleanCap Reagent AU was specifically designed for self-replicating RNAs based on the genomes of positive sense strand RNA viruses such as Venezuelan equine encephalitis virus (VEEV), Semliki forest virus (SFV) and Sindbis virus (SIN). The positive sense strand genomes of these viruses start with a 5'-AU... Cap 1 mRNAs have superior in vivo activity compared to Cap 0 mRNA produced by legacy cotranscriptional capping methods anti-reverse cap analog (ARCA). CleanCap can be used in conjunction with TriLink's catalog of modified NTPs.
Product Details
Catalog NoN-7114
Purity≥95% by AX-HPLC
Extinction Coefficient33,400 Lmol-1cm-1 at 258 nm
Molecular FormulaC31H42N12O25P4 (free acid)
Molecular Weight1106.6 g/mole (free acid)
Salt FormNa+
Concentration100 mM
BufferH2O
Recommended Storage-20°C or below
Other Name(s)CleanCap® AU for Self-Amplifying mRNA, CleanCap® Reagent AU for co-transcriptional capping of mRNA, m7G(5')ppp(5')(2'OMeA)pU
ApplicationCRISPR, In vitro Transcription, Recombinases, TALENS, Transposases, Vaccine Development, Zinc Finger Nucleases
Backbone5'-5'-Triphosphate
Base Analog(s)Adenosine, Uridine
Sugar Type(s)RNA
Nucleotide CategoryCap analogs