核酸转染增强试剂——NATE™

InvivoGen 新品推荐：核酸转染增强试剂——NATE™

产品介绍：

NATE™是InvivoGen设计的一种核酸转染增强剂，可以提高难转染细胞的瞬转与稳转效率，尤其适合人的单核细胞与小鼠巨噬细胞等难转染的细胞，如外周血单核细胞（THP-1）和小鼠单核巨噬细胞白血病细胞（RAW 264.7），且只需在平时加转染试剂操作前30min加入NATE™即可。值得注意的是，NATE对细胞温和，不会对细胞培养产生任何进一步的毒性。

在真核细胞转染过程中，外源性核酸(如质粒)的主要障碍是会被胞质传感器检测，如cGAS/STING、AIM2炎性小体和LC3介导的自噬。这些防御信号级联的激活常常会降低转染率和细胞存活率，特别是在难以转染的细胞(如免疫细胞)中会更明显。当使用NATE™时，这些核酸传感通路将被抑制，从而在转染过程中保护质粒并促进其表达。

产品特色：

● 与常用转染试剂 (e.g. GeneXPlus, Lipofectamine® LTX, and jetPRIME®) 及物理方法兼容。

● 更高的转染率，也适用于大质粒 (> 10kb)。

● 在所有的转染测试方案中都显示对细胞温和，没有毒性。

产品信息：

应用举例：

Top - Transient transfection of an ~3 kb GFP-expressing plasmid into THP-1 cells was performed using GeneXPlus both in the absence (left) and presence (right) of NATE™. After 48 hours, the transfected cells expressing GFP were visualized by fluorescence microscopy. Bottom - Transient transfections of an ~3 kb GFP-expressing plasmid (left) and an 8 kb GFP-expressing plasmid (right) into THP-1 cells was performed using commonly used transfection methods such as GeneXPlus, Lipofectamine® LTX, jetPRIME®, and nucleofection. This was performed both in the presence (green) and absence (yellow) of NATE™. After 48 hours, transfection efficiency (% GFP-expressing cells) was measured using flow cytometry. Data are presented as a fold change compared to transfection without NATE™ 

Top - Transient transfection of an ~3 kb GFP-expressing plasmid into RAW 264.7 cells was performed using Lipofectamine® LTX both in the absence (left) and presence (right) of NATE™. After 48 hours, the transfected cells expressing GFP were visualized by fluorescence microscopy. Bottom - Transient transfection of an ~3 kb GFP-expressing plasmid (left) and an 8 kb GFP-expressing plasmid (right) into RAW 264.7 cells was performed using commonly used transfection methods including Lipofectamine® LTX, jetPRIME®, FuGENE®, and nucleofection. This was performed both in the presence (green) and absence (yellow) of NATE™. After 48 hours, transfection efficiency (% GFP-expressing cells) was measured using flow cytometry. Data are presented as fold change compared to transfection without NATE™.

Stable transfection of an ~10 kb SEAP‑expressing plasmid into RAW 264.7 cells was performed using Lipofectamine® LTX both in the absence (left) and presence (right) of NATE™. After 10 days in selection with Blasticidin, the number of stable clones expressing SEAP (blue wells) was visualized using QUANTI‑Blue™, a SEAP detection reagent.

详情请联系 invivogen 中国授权代理-欣博盛生物科技