Enzo Life Sciences：ROS-ID®总活性氧/超氧化物检测试剂盒

Enzo Life Sciences的ROS-ID® Total ROS/Superoxide detection kit包括两种荧光染料作为主要成分：非荧光、可渗透细胞的氧化应激检测试剂（绿色，Ex/Em 490/525 nm）与多种活性物质（过氧化氢、过氧亚硝酸根和羟基）直接反应，产生绿色荧光产物。超氧化物检测试剂（橙色，Ex/Em 550/620 nm）是一种可渗透细胞的探针，可与超氧化物特异性反应，产生橙色荧光产物。试剂盒还提供Pycyanin（ROS诱导剂）和NAC（ROS抑制剂）。

产品特点

● 用于检测活细胞中超氧化物的产生

● 与细胞培养基的主要成分（酚红，FBS和BSA）兼容

● 已通过显微镜、流式细胞仪和微孔板等应用验证

实验示例

图1. 在加入了ROS/超氧化物检测试剂并用绿脓菌素处理的 HeLa 细胞中，通过荧光显微镜对活性氧的形成进行了分析。在绿色通道中监测一般氧化应激水平，而在橙色通道中检测超氧化物的产生。用 NAC（一种通用的 ROS 抑制剂）预处理可防止 ROS 的形成。

图2. 使用ROS-ID® Total ROS/Superoxide detection kit和ROS清除剂/抑制剂分析用抗-霉-素A（AMA，特异性超氧化物诱导剂）、叔丁基-过氧化物（TBHP，特异性过氧化物诱导剂）和绿脓菌素（通用ROS诱导剂）处理的HeLa细胞中ROS的产生。使用U-2 OS和CHO K1细胞获得了类似的结果。

图3. 通过流式细胞术分析Hela细胞中ROS的形成。数据以绿脓菌素（ROS/SO诱导剂）、TBHP（ROS 诱导剂）和 AMA（超氧化物诱导剂）处理后的阳性百分比表示。绿色柱表示ROS 阳性率%，橙色柱表示超氧化物阳性率%。

产品信息

部分产品引用文献

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3. Eicosatetraynoic Acid and Butyrate Regulate Human Intestinal Organoid Mitochondrial and Extracellular Matrix Pathways Implicated in Crohn’s Disease Strictures: I. Jurickova, et al.; Inflamm. Bowel Dis. 28, 988 (2022)

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6. New glycoconjugation strategies for Ruthenium(II) arene complexes via phosphane ligands and assessment of their antiproliferative activity: D. Iacopini, et al.; Bioorg. Chem. 126, 105901 (2022)

7. Quercetin treatment protects the Achilles tendons of rats from oxidative stress induced by hyperglycemia: T. Yoshikawa, et al.; BMC Musculoskelet. Disord. 23, 563 (2022)

8. Areca nut extract (ANE) inhibits the progression of hepatocellular carcinoma cells via activation of ROS production and activation of autophagy: P.L. Wei, et al.; Int. J. Med. Sci. 18, 3452 (2021)

9. Differential oxidative stress responses and tobacco-specific nitrosamine accumulation in two burley varieties: J. Kurepa, et al.; J. Plant Physiol. 261, 153429 (2021)

10. Dihydroisotanshinone I induced ferroptosis and apoptosis of lung cancer cells: C.Y. Wu, et al.; Biomed. Pharmacother. 139, 111585 (2021)

11. Incense smoke-induced oxidative stress disrupts tight junctions and bronchial epithelial barrier integrity and induces airway hyperresponsiveness in mouse lungs: N. Yamamoto, et al.; Sci. Rep. 11, 7222 (2021)

12. Nano-encapsulation of hydroxytyrosol into formulated nanogels improves therapeutic effects against hepatic steatosis: An in vitro study: E. Mauri, et al.; Mater. Sci. Eng. C Mater. Biol. Appl. 124, 112080 (2021)

13. Non-viral gene delivery of the oncotoxic protein NS1 for treatment of hepatocellular carcinoma: D. Witzigmann, et al.; J. Control Release 334, 138 (2021)

14. Polymer-ritonavir derivate nanomedicine with pH-sensitive activation possesses potent anti-tumor activity in vivo via inhibition of proteasome and STAT3 signaling: L. Sivák, et al.; J. Control Release 332, 563 (2021)

15. Selective striatal cell loss is ameliorated by regulated autophagy of the cortex: K. Cho & G.W. Kim; Life Sci. 282, 119822 (2021)

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