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Enzo Life Sciences:ROS-ID®总活性氧/超氧化物检测试剂盒

更新时间:2024-02-04   点击次数:156次

Enzo Life Sciences的ROS-ID® Total ROS/Superoxide detection kit包括两种荧光染料作为主要成分:非荧光、可渗透细胞的氧化应激检测试剂(绿色,Ex/Em 490/525 nm)与多种活性物质(过氧化氢、过氧亚硝酸根和羟基)直接反应,产生绿色荧光产物。超氧化物检测试剂(橙色,Ex/Em 550/620 nm)是一种可渗透细胞的探针,可与超氧化物特异性反应,产生橙色荧光产物。试剂盒还提供Pycyanin(ROS诱导剂)和NAC(ROS抑制剂)。


ROS-ID®总活性氧/超氧化物检测试剂盒——ENZO热销产品

 

产品特点

● 用于检测活细胞中超氧化物的产生

● 与细胞培养基的主要成分(酚红,FBS和BSA)兼容

● 已通过显微镜、流式细胞仪和微孔板等应用验证

 

实验示例

ROS-ID®总活性氧/超氧化物检测试剂盒——ENZO热销产品

图1. 在加入了ROS/超氧化物检测试剂并用绿脓菌素处理的 HeLa 细胞中,通过荧光显微镜对活性氧的形成进行了分析。在绿色通道中监测一般氧化应激水平,而在橙色通道中检测超氧化物的产生。用 NAC(一种通用的 ROS 抑制剂)预处理可防止 ROS 的形成。

 

ROS-ID®总活性氧/超氧化物检测试剂盒——ENZO热销产品

图2. 使用ROS-ID® Total ROS/Superoxide detection kit和ROS清除剂/抑制剂分析用抗-霉-素A(AMA,特异性超氧化物诱导剂)、叔丁基-过氧化物(TBHP,特异性过氧化物诱导剂)和绿脓菌素(通用ROS诱导剂)处理的HeLa细胞中ROS的产生。使用U-2 OS和CHO K1细胞获得了类似的结果。

ROS-ID®总活性氧/超氧化物检测试剂盒——ENZO热销产品

图3. 通过流式细胞术分析Hela细胞中ROS的形成。数据以绿脓菌素(ROS/SO诱导剂)、TBHP(ROS 诱导剂)和 AMA(超氧化物诱导剂)处理后的阳性百分比表示。绿色柱表示ROS 阳性率%,橙色柱表示超氧化物阳性率%。

 

 

产品信息

产品货号

ENZ-51010

产品名称

ROS-ID® Total ROS/Superoxide detection kit/欣博盛生物

别名

Reactive oxygen species / Superoxide

规格

1*1Kit

(200 fluorescence microscopy assays or 50 flow cytometry assays or 2x96 microplate assays)

使用/稳定性

With proper storage, the kit components are stable up to the date noted on the product label. 

Store kit at -20°C in a non-frost free freezer, or -80°C for longer term storage.

短期保存

-20°C

长期保存

-80°C

试剂盒组分

Oxidative Stress Detection Reagent (Green), 300 nmoles

Superoxide Detection Reagent (Orange), 300 nmoles

ROS Inducer (Pyocyanin), 1 µmole

ROS Inhibitor (N-acetyl-L-cysteine), 2 x 10 mg

Wash Buffer Salts, 1 pack

应用

Flow Cytometry, Fluorescence microscopy, Fluorescent detection, HTS

 

部分产品引用文献

1. Conditional Knockout of Hypoxia-Inducible Factor 1-Alpha in Tumor-Infiltrating Neutrophils Protects against Pancreatic Ductal Adenocarcinoma: J.L. Sieow, et al.; Int. J. Mol. Sci. 24, 753 (2023)

2. Antioxidant effect of nicotinamide mononucleotide in tendinopathy: K. Yamaura, et al.; BMC Musculoskelet. Disord. 23, 249 (2022)

3. Eicosatetraynoic Acid and Butyrate Regulate Human Intestinal Organoid Mitochondrial and Extracellular Matrix Pathways Implicated in Crohn’s Disease Strictures: I. Jurickova, et al.; Inflamm. Bowel Dis. 28, 988 (2022)

4. Hirsutella sinensis mycelium attenuates bleomycin-induced pulmonary inflammation and fibrosis in vivo: T.T. Huang, et al.; Sci. Rep. 5, 15282 (2022)

5. Inhibition of mitoNEET attenuates LPS-induced inflammation and oxidative stress: S. Lee, et al.; Cell Death Dis. 13, 127 (2022)

6. New glycoconjugation strategies for Ruthenium(II) arene complexes via phosphane ligands and assessment of their antiproliferative activity: D. Iacopini, et al.; Bioorg. Chem. 126, 105901 (2022)

7. Quercetin treatment protects the Achilles tendons of rats from oxidative stress induced by hyperglycemia: T. Yoshikawa, et al.; BMC Musculoskelet. Disord. 23, 563 (2022)

8. Areca nut extract (ANE) inhibits the progression of hepatocellular carcinoma cells via activation of ROS production and activation of autophagy: P.L. Wei, et al.; Int. J. Med. Sci. 18, 3452 (2021)

9. Differential oxidative stress responses and tobacco-specific nitrosamine accumulation in two burley varieties: J. Kurepa, et al.; J. Plant Physiol. 261, 153429 (2021)

10. Dihydroisotanshinone I induced ferroptosis and apoptosis of lung cancer cells: C.Y. Wu, et al.; Biomed. Pharmacother. 139, 111585 (2021)

11. Incense smoke-induced oxidative stress disrupts tight junctions and bronchial epithelial barrier integrity and induces airway hyperresponsiveness in mouse lungs: N. Yamamoto, et al.; Sci. Rep. 11, 7222 (2021)

12. Nano-encapsulation of hydroxytyrosol into formulated nanogels improves therapeutic effects against hepatic steatosis: An in vitro study: E. Mauri, et al.; Mater. Sci. Eng. C Mater. Biol. Appl. 124, 112080 (2021)

13. Non-viral gene delivery of the oncotoxic protein NS1 for treatment of hepatocellular carcinoma: D. Witzigmann, et al.; J. Control Release 334, 138 (2021)

14. Polymer-ritonavir derivate nanomedicine with pH-sensitive activation possesses potent anti-tumor activity in vivo via inhibition of proteasome and STAT3 signaling: L. Sivák, et al.; J. Control Release 332, 563 (2021)

15. Selective striatal cell loss is ameliorated by regulated autophagy of the cortex: K. Cho & G.W. Kim; Life Sci. 282, 119822 (2021)



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详情请联系Enzo Life Sciences金牌代理——欣博盛生物