Enzo Life Sciences：ROS-ID®活性氧/活性氮检测试剂盒

自由基和其他活性种在许多心理和病理生理过程中发挥着重要作用。自由基一旦在细胞内产生，就会破坏多种细胞成分，包括蛋白质、脂质和DNA。然而，在较低浓度下，自由基可以作为细胞信号传导中的第二信使。

Enzo Life Sciences/欣博盛生物的ROS-ID® ROS/RNS detection kit可用荧光显微镜直接实时监测活细胞中活性氧与活性氮（ROS/RNS）的产生。该试剂盒中包含三种检测探针：NO检测试剂（红色，Ex/Em 650/670 nm），用于总ROS检测的氧化应激检测试剂（绿色，Ex/Em 490/525 nm）和超氧化物检测试剂（橙色，Ex/Em 550/620 nm）。试剂盒内还包括阳性对照，绿脓菌素和L-精氨酸，它们分别是ROS和NO产生的常见诱导剂；阴性对照N-乙酰-L-半胱氨酸和c-PTIO则分别是ROS和NO的常见清除剂。通过将三种检测探针与一组特异性抑制剂、激活剂相结合，能够区分超氧化物、一氧化氮和过氧亚硝酸盐。

产品特点

● 直接监测活细胞中的活性氧和氮化物（ROS/RNS）

● 可区分超氧化物、一氧化氮和过氧亚硝酸盐

● 高灵敏度、特异性和准确性，可用于活细胞研究

● 与组织培养基的主要成分（酚红、FBS和BSA）兼容

● 试剂盒内包含整套试剂，包括ROS/RNS诱导剂和清除剂

实验示例

图1. 氧化应激检测试剂（绿色）、超氧化物检测试剂（蓝色）和NO检测试剂（红色）的激发和发射光谱。

图2. 使用ROS/RNS检测试剂盒演示HeLa细胞中过氧亚硝酸盐和NO生成的代表性实验。分析表明，由于NO与超氧化物的反应，L-精氨酸和绿脓菌素的联合处理产生过氧亚硝酸盐（绿色荧光），但很少产生NO（红色荧光）。

产品信息

部分产品引用文献

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8. Isoorientin derived from Gentiana veitchiorum Hemsl. flowers inhibits melanogenesis by down-regulating MITF-induced tyrosinase expression: Q. Wu, et al.; Phytomedicine 57, 129 (2019)

9. Low intensity ultrasound reduces high glucose reduced nitric oxide generation in retinal pigment epithelial cells: M.B. Karmacharya, et al.; Ultrasound Med. Biol. 44, 647 (2018)

10. HEK-293 secretome attenuates kainic acid neurotoxicity through insulin like growth factor-phosphatidylinositol-3-kinases pathway and by temporal regulation of antioxidant defense machineries: C. Venugopal, et al.; Neurotoxicology 69, 189 (2017)

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12. Cell-to-cell diffusion of glucose in the mammalian heart is disrupted by high glucose. Implications for the diabetic heart: W.C. De Mello ; Exp. Cell Res. 334, 239 (2015)

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14. Regulation of gene expression by tobacco product preparations in cultured human dermal fibroblasts: G.E. Malpass, et al.; Toxicol. Appl. Pharmacol. 279, 211 (2014)

15. Enterococcus faecalis infection causes inflammation, intracellular oxphos-independent ROS production, and DNA damage in human gastric cancer cells: J.A. Strickertsson, et al.; PLoS One 8, e63147 (2013)

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