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ENZO热销产品——LTB4 ELISA kit,白三烯B4检测试剂盒

更新时间:2025-01-23   点击次数:109次

白三烯(Leukotriene, LT)是花生四烯酸的5-脂氧合酶代谢的主要产物,能够刺激白细胞的功能,包括溶酶体酶的释放、粘附和多形核白细胞(即嗜中性、嗜碱性和嗜酸性白细胞)的聚集,被认为是炎症性疾病和免疫调节的强效介质。半胱氨酰白三烯(LTC4、LTD4和LTE4)是通过将半胱氨酸衍生物加到LTA4上形成的,LTC4合酶将LTA4转化为LTC4限制了半胱氨酰白三烯的形成速率。半胱氨酰白三烯作为炎症中的强效脂质介质,可诱导平滑肌收缩和毛细血管通透性增加。

Enzo Life Sciences的高品质热销产品LTB4 ELISA kit(货号:ADI-900-068)具有超高灵敏度和高文献引用率,批次间差异小,上市多年广受用户好评。

 

产品亮点:

● 超灵敏度,对LTB4的超灵敏测量,检测浓度低至5.63 pg/mL;

● 高文献引用,在众多同行评审的出版物中被广泛引用;

● 宽动态范围,适用于多种样本类型;

● 高通量格式,做复孔的情况下,可在4小时内获得多达39份样本的结果;

● 批间批内差异小,检测结果具有高度的可重复性;

● 完-全定量结果,优于WB的半定量分析。

 

产品参数:

产品名称

LTB4 ELISA kit

别名

Leukotriene B4 ELISA kit

产品货号

ADI-900-068 / ADI-901-068

规格

1*96 wells/ 5*96 wells

应用

Colorimetric detection, ELISA

应用笔记

For the quantitative determination of LTB4 in culture supernatants, 

plasma, saliva, and urine from any species. Cited sample types 

include bronchioalveolar lavage fluid, cell lysate, crevicular fluid, 

tissue, serum and whole blood.

分析时长

4 hours

产品组分

GxR IgG Microtiter plate, Conjugate, Antibody, Assay buffer, Wash 

buffer concentrate, Standard, pNpp Substrate, Stop solution

灵敏度

5.63 pg/ml

检测范围

11.7 - 3,000 pg/ml

反应物种

Species independent

检测波长

405 nm

使用/稳定性

Store all components at +4°C, except conjugate at -20°C.

运输条件

Blue Ice

 

高分文献引用:

近期,Adams发表了题为《Leukotriene A4 hydrolase inhibition improves age-related cognitive decline via modulation of synaptic function》的文章,在该项研究中,Adams等人使用了Enzo的LTB4 ELISA试剂盒检测小鼠血浆,测定了老年小鼠、给药白三烯A4水解酶抑制剂(LTA4H)的小鼠体内炎症介质白三烯B4(LTB4)水平,证明了LTA4H抑制剂可以减少LTB4的产生,并改善小鼠与年龄相关的认知能力下降。

LTB4 ELISA.jpg

 

(A) Plasma LTB4 measured from unstimulated blood is significantly increased from 3 to 22.5 months (M). Unpaired t test, **P = 0.0011, F = 2.952 (n = 15 and 11). (B) Plasma LTB4 levels from calcimycin-stimulated blood are significantly reduced following 4 weeks of dosing with LTA4H inhibitors AKST1220 or SC57461A. Kruskal-Wallis test, P = 0.0006 followed by Dunn’s multiple comparisons: vehicle versus AKST1220, **P = 0.0034; vehicle versus SC57461A, *P = 0.0212; and AKST1220 versus SC57461A, P > 0.9999 (n = 5, 6, and 9).

 

Adams团队对LTB4 ELISA kit的真实评价:

Q: Why did you use Enzo’s LTB4 ELISA Kit?

A: Species-independent measurement of LTB4 in culture supernatant, plasma, saliva, urine, and other cited sample types.

Q: How do you position Enzo’s LTB4 ELISA Kit?

A: The LTB4 ELISA kit is a colorimetric competitive enzyme immunoassay kit with results in 4 hours. It is designed to assay up to 39 samples in duplicate with a broad dynamic range that is suitable for a wide variety of sample matrices, including bronchioalveolar lavage fluid, cell lysate, crevicular fluid, tissue, serum and whole blood.

 

LTB4 ELISA kit最新引用文献:

1.  Leukotriene A4 hydrolase inhibition improves agerelated cognitive decline via modulation of synaptic function: J.M. Adams, et al.; Sci. Adv. 9, eadf8764 (2023), Abstract

2. Infection of Galleria mellonella (lepidoptera) larvae with the entomopathogenic fungus Conidiobolus coronatus (entomophthorales) induces apoptosis of hemocytes and affects the concentration of eicosanoids in the hemolymph: A.K. Wronska, et al.; Front. Physiol. 12, 774086 (2022), Application(s): ELISA kit using hemolymph, Abstract — Full Text

3.  Neobavaisoflavone inhibits allergic inflammatory responses by suppressing mast cell activation: R. Liang, et al.; Int. Immunopharmacol. 110, 108953 (2022), Abstract

4. Effect of Dysglycemia on Urinary Lipid Mediator Profiles in Persons With Pulmonary Tuberculosis: M.B. Arriaga, et al.; Front. Immunol. 13, 919802 (2022), Abstract

 

Enzo Life Sciences总部设于美国纽约,在研发、生产、销售生物研究试剂方面拥有40多年的成功经验,可提供多种试剂盒、蛋白、抗体、多肽、小分子、标签探针染料等产品,用于研究靶标鉴定及验证、高通量分析、基因表达分析、蛋白质检测及细胞分析等相关领域。

 


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