更新时间:2022-01-28
CpG oligonucleotides (ODNs) are synthetic ODNs that contain unmethylated CpG dinucleotides in specific sequence contexts (CpG motifs).These CpG motifs are present at a 20-fold greater frequency in ……
CpG oligonucleotides (ODNs) are synthetic ODNs that contain unmethylated CpG dinucleotides in specific sequence contexts (CpG motifs).
These CpG motifs are present at a 20-fold greater frequency in bacterial DNA than in mammalian DNA. CpG ODNs activate Toll-like receptor 9 (TLR9), leading to strong immunostimulatory effects.
ODN 2006 (ODN 7909) is a class B CpG ODN and is a ligand of choice for human TLR9. Class B CpG ODNs contain a full phosphorothioate backbone with one or more CpG dinucleotides.
They strongly activate B cells but weakly stimulate IFN-α secretion in pDCs.
Synonyms: ODN 7909, PF_3512676
Specificity: human TLR9 agonist
Working concentration: 1-5 µM
Solubility: 5 mg/ml in water
ODN 2006 sequence:
5’-tcgtcgttttgtcgttttgtcgtt-3’ (24 mer)
Note: Bases are phosphorothioate (nuclease resistant).
Quality control:
- TLR9 activity has been tested using HEK-Blue™ TLR9 cells.
- The absence of bacterial contamination (e.g. lipoproteins and endotoxins) has been confirmed using HEK-Blue™ TLR2 and HEK-Blue™ TLR4 cells.
ODN2006 is provided lyophilized and is available in three quantities:
tlrl-2006 (formerly tlrl-hodnb):
200 μg (25.96 nmol) lyophilized ODN2006
1.5 ml sterile endotoxin-free water
tlrl-2006-1 (formerly tlrl-hodnb-1):
1 mg (129.8 nmol) lyophilized ODN2006
1.5 ml sterile endotoxin-free water
tlrl-2006-5 (formerly tlrl-hodnb-5):
5 mg (649 nmol) lyophilized ODN2006
10 ml sterile endotoxin-free water
ODN 2006 (ODN 7909) is shipped at room temperature.
Upon receipt, store at -20 °C.
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