CleanCap® Reagent AG - (N-7113)

CleanCap® Reagent AG is TriLink’s proprietary co-transcriptional capping reagent for in vitro transcription of 5’ capped mRNA resulting in a Cap 1 structure. CleanCap has shown to provide up to 98% capping efficiency. PLEASE NOTE: TriLink recommends using CleanCap AG whenever possible. CleanCap AG requires an AG initiator. CleanCap AG typically provides >90% capped material generating a naturally occurring Cap 1 structure. Unlike CleanCap AG, using CleanCap GG (cat. no. N-7133 or N-7433) may result in comparable ARCA transcriptional stuttering and yield.

Product Details

Catalog NoN-7113

Purity≥95% by AX-HPLC

Extinction Coefficient30,539 Lmol-1cm-1 at 255 nm

Molecular FormulaC32H43N15O24P4 (free acid)

Molecular Weight1145.60 g/mole (free acid)

Salt FormNa+

Concentration100 mM

BufferH2O

Recommended Storage-20°C or below

Other Name(s)CleanCap® Reagent AG for co-transcriptional capping of mRNA,m7G(5')ppp(5')(2'OMeA)pG

ApplicationCRISPR, In vitro Transcription, Recombinases, TALENS, Transposases, Vaccine Development, Zinc Finger Nucleases

Backbone5'-5'-Triphosphate

Base Analog(s)Adenosine, Guanosine

Nucleotide CategoryCap analogs