Highly sensitive measurement, detecting as little as 13.4 pg/ml PGE2
Higher throughput format with results in <3 hrs for up to 37 samples in duplicate
Widely cited in peer reviewed literature
Ready-to-use liquid color-coded reagents reduce errors
Reproducible results day-after-day and lot-to-lot
The PGE2 EIA kit is a colorimetric competitive enzyme immunoassay kit with results in < 3 hours. Absorbance is read at 405 nm. Screen inhibitors of COX II activity by measuring the levels of downstream PGE2. Commercially available since 1992, this kit is widely cited in peer-reviewed publications. The non-radioactive ready-to-use liquid color-coded reagents reduce errors.
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Product Details
| Alternative Name: | Prostaglandin E2 |
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| Sensitivity: | 13.4 pg/ml (range 39.1 - 2,500 pg/ml) |
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| Assay Time: | <3 hours |
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| Applications: | ELISA, Colorimetric detection
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| Application Notes: | For the quantitative determination of PGE2 in culture supernatants, serum, saliva, urine, and whole blood from any species. Cited sample types include cerebral spinal fluid, dialysate, gingival crevicular fluid, peritoneal exudate fluid and peritoneal exudate cell supernatant, plasma, and tissue. |
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| Wavelength: | 405 nm |
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| Species reactivity: | Species independent
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| Use/Stability: | Store all components at +4°C, except standard and conjugate at -20°C. |
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| Shipping: | Shipped on Blue Ice |
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| Contents: | GxM IgG Microtiter plate, Conjugate, Antibody, Assay buffer, Wash buffer concentrate, Standard, pNpp Substrate, Stop solution |
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| Scientific Background: | Prostaglandin E2 (PGE2) is an extensively studied prostaglandin owing to its predominance in inflammation, cancer, atherosclerosis, autoimmune disease, and sepsis. Oxidation of arachidonic acid by prostaglandin synthases (COX-1 and COX-2) produces prostaglandin H2 (PGH2), which is further metabolized by PGE synthases into its major product, PGE2. PGE2 mediates autocrine and paracrine signaling by binding to G-protein coupled receptors (EP1, EP2, EP3, EP4) on the cell surface, functioning to modulate phospholipase C and adenylate cyclase activity. PGE2 has been of great interest as a therapeutic target, either by modulation of its synthesis by COX inhibitors (NSAIDS) or by modulation of its receptors by downregulation or binding antagonists. PGE2 production in a variety of tissues has been shown to modulate numerous physiological processes including natriuresis in the kidney, smooth muscle elasticity in the vasculature, and the inflammatory response to damaged tissues by monocytes and macrophages. |
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| Regulatory Status: | RUO - Research Use Only |
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| Compatibility: | This product is compatible with the Absorbance 96 Plate Reader. |
Product Literature References
A Novel Class of Dual-Acting DCH-CORMs Counteracts Oxidative Stress-Induced Inflammation in Human Primary Tenocytes: F. Appetecchia, et al.; Antioxidants 10, 1828 (2021), Abstract;
Antioxidants and/or fish oil reduce intermittent hypoxia-induced inflammation in the neonatal rat terminal ileum: G. Mustafa, et al.; Prostaglandins Other Lipid Mediat. 155, 106565 (2021), Abstract;
Discovery of N-amido-phenylsulfonamide derivatives as novel microsomal prostaglandin E 2 synthase-1 (mPGES-1) inhibitors: M. Kim, et al.; Bioorg. Med. Chem. Lett. 41, 127992 (2021), Abstract;
Effect of mesenchymal stem cells combined with chondroitin sulfate in an in vitro model of osteoarthritis: S. Pérez-Castrillo, et al.; Am. J. Transl. Res. 13, 5928 (2021), Abstract;
Leptin reduces in vitro cementoblast mineralization and survival as well as induces PGE2 release by ERK1/2 commitment: G. Ruiz-Heiland, et al.; Clin. Oral. Investig. 25, 1933 (2021), Abstract; Full Text
Potential Surrogate Quantitative Immunomodulatory Potency Assay for Monitoring Human Umbilical Cord-derived Mesenchymal Stem Cells Production: S. Teng, et al.; Cell Biol. Int. 45, 1072 (2021), Abstract;
CoCrMo surface modifications affect biocompatibility, adhesion, and inflammation in human osteoblasts: B. Lohberger, et al.; Sci. Rep. 10, 1682 (2020), Abstract; Full Text
Genetic deletion of connexin 37 causes polyuria and polydipsia: J. Xue, et al.; PLoS One 15, e0244251 (2020), Abstract; Full Text
Hypoxia-preconditioned mesenchymal stem cells prevent renal fibrosis and inflammation in ischemia-reperfusion rats: N. Ishiuchi, et al.; Stem Cell Res. Ther. 11, 130 (2020), Abstract; Full Text
Interleukin-1 receptor-induced PGE2 production controls acetylcholine-mediated cardiac dysfunction and mortality during scorpion envenomation: M.B. Reis, et al.; Nat. Commun. 11, 5433 (2020), Abstract; Full Text
Apolipoprotein D overexpression alters hepatic prostaglandin and omega fatty acid metabolism during the development of a non-inflammatory hepatic steatosis: F. Desmarais, et al.; Biochim. Biophys. Acta Mol. Cell Biol. Lipids 1864, 522 (2019), Application(s): ELISA using liver homogenates, Abstract;
Baltic amber teething necklaces: could succinic acid leaching from beads provide anti-inflammatory effects?: M.D. Nissen, et al.; BMC Complement. Altern. Med. 19, 162 (2019), Abstract;
IRE1α-XBP1 signaling in leukocytes controls prostaglandin biosynthesis and pain: S. Chopra, et al.; Science 365, eaau6499 (2019), Application(s): Cell Culture Supernatant, Abstract;
Mechanisms utilized by feline adipose-derived mesenchymal stem cells to inhibit T lymphocyte proliferation: N. Taechangam, et al.; Stem Cell Res. Ther. 10, 188 (2019), Abstract; Full Text
Molecular profiling and functional analysis of macrophage-derived tumor extracellular vesicles: C. Cianciaruso, et al.; Cell Rep. 27, 3062 (2019), Application(s): ELISA using culture supernatants, Abstract; Full Text
Olive-Derived Hydroxytyrosol Shows Anti-inflammatory Effect without Gastric Damage in Rats: Y. Yonezawa, et al.; Biol. Pharm. Bull. 42, 1120 (2019), Abstract;
Redox Control of IL-6-Mediated Dental Pulp Stem-Cell Differentiation on Alginate/Hydroxyapatite Biocomposites for Bone Ingrowth: S. Sancilio, et al.; Nanomaterials (Basel) 9, 1656 (2019), Abstract; Full Text
The serine protease inhibitors and SERPINE1/2 disrupt prostaglandin E2 production and hyaluronic acid retention in FSH‐stimulated pig cumulus–oocyte complexes: M. Blaha, et al.; Reprod. Domest. Anim. 54, 65 (2019), Application(s): Cell culture supernatant from porcine cumulus-oocyte complexes, Abstract;
An ethanol extract of the rhizome of Atractylodes chinensis exerts anti-gastritis activities and inhibits Akt/NF-κB signaling: M.J. Hossen, et al.; J. Ethnopharmacol. 228, 18 (2018), Abstract;
Anti-neuroinflammatory effects of GPR55 antagonists in LPS-activated primary microglial cells: S.W. Saliba, et al.; J. Neuroinflammation 15, 322 (2018), Abstract; Full Text
Carbon monoxide ameliorates Staphylococcus aureus-elicited COX-2/IL-6/MMP-9-dependent human aortic endothelial cell migration and inflammatory responses: M.H. Tsai, et al.; Immunol. Lett. 203, 40 (2018), Abstract;
Carbon monoxide releasing molecule-2 attenuates Pseudomonas aeruginosa-induced ROS-dependent ICAM-1 expression in human pulmonary alveolar epithelial cells: C.W. Lee, et al.; Redox Biol. 18, 93 (2018), Abstract; Full Text
Dietary Supplementation With ω6 LC-PUFA-Rich Algae Modulates Zebrafish Immune Function and Improves Resistance to Streptococcal Infection: S. Nayak, et al.; Front. Immunol. 9, 1960 (2018), Application(s): Zebrafish kidney lysates, Abstract; Full Text
Endogenous metabolites of vitamin E limit inflammation by targeting 5-lipoxygenase: H. Pein, et al.; Nat. Commun. 9, 3834 (2018), Application(s): Peritoneal exudates, Abstract; Full Text
Ketoprofen affects the mammary immune response in dairy cows in vivo and in vitro: D. Dan, et al.; J. Dairy Sci. 101, 11321 (2018), Abstract;
Prostaglandin E2 secreted from feline adipose tissue-derived mesenchymal stem cells alleviate DSS-induced colitis by increasing regulatory T cells in mice: J.H. An, et al.; BMC Vet. Res. 14, 354 (2018), Abstract; Full Text
Synthetic 3',4'-Dihydroxyflavone Exerts Anti-Neuroinflammatory Effects in BV2 Microglia and a Mouse Model: N. Kim, et al.; Biomol. Ther. (Seoul) 26, 210 (2018), Abstract; Full Text
Anti-inflammatory effect of lycopene in SW480 human colorectal cancer cells: J.H. Cha, et al.; Nutr. Res. Pract. 11, 90 (2017), Abstract; Full Text
Colon cancer-derived myofibroblasts increase endothelial cell migration by glucocorticoid-sensitive secretion of a pro-migratory factor: Z. Drebert, et al.; Vascul. Pharmacol. 89, 19 (2017), Application(s): Determine the concentrations of prostanoids in conditioned medium from myofibroblasts and HUVECs, and in HUVEC lysates, Abstract;
Differential Effects of sEH Inhibitors on the Proliferation and Migration of Vascular Smooth Muscle Cells: H.S. Kim, et al.; Int. J. Mol. Sci. 18, 2683 (2017), Abstract; Full Text
Expression of toll-like receptor-1 by bradykinin in human gingival fibroblasts: J.A. Arreguin-Cano T; Int. J. Clin. Exp. Pathol. 10, 1283 (2017), Application(s): Measurement of PGE2 in supernatant., Full Text
Human and feline adipose-derived mesenchymal stem cells have comparable phenotype, immunomodulatory functions, and transcriptome: H.C. Clark, et al.; Stem Cell Res. Ther. 8, 69 (2017), Application(s): Measurement of PGE2 in supernatant of human and feline cells., Abstract; Full Text
Inhibitory effects of Aconiti Lateralis Radix Preparata on chronic intermittent cold-induced inflammation in the mouse hypothalamus: W. Kim, et al.; J. Ethnopharmacol. 215, 27 (2017), Abstract;
Mast cell degranulation and calcium influx are inhibited by an Echinacea purpurea extract and the alkylamide dodeca-2E, 4E-dienoic acid isobutylamide: T.V. Gulledge, et al.; J. Ethnopharmacol. 212, 166 (2017), Abstract;
Monoacylglycerol lipase inhibitor JZL184 prevents HIV-1 gp120-induced synapse loss by altering endocannabinoid signaling: X. Zhang, et al.; Neuropharmacology 128, 269 (2017), Abstract;
Novel augmentation by bufalin of protein kinase C-induced cyclooxygenase-2 and IL-8 production in human breast cancer cells: H.T. Chen, et al.; Innate Immun. 23, 54 (2017), Abstract; Full Text
Prostaglandin E2 stimulates the expression of cumulus expansion-related genes in pigs: the role of protein kinase B: M. Blaha, et al.; Prostaglandins Other Lipid Mediat. 130, 38 (2017), Application(s): ELISA using pig samples, Abstract;
The effect of intra-articular botulinum toxin A on substance P, prostaglandin E2, and tumor necrosis factor alpha in the canine osteoarthritic joint: H.M. Heikkilä, et al.; BMC Vet. Res. 13, 74 (2017), Application(s): Canine synovial fluid and serum, Abstract; Full Text
A positive feedback loop between progesterone and microsomal prostaglandin E synthase-1-mediated PGE2 promotes production of both in mouse granulosa cells: K. Tamura, et al.; Prostaglandins Other Lipid Mediat. 123, 56 (2016), Application(s): Measurement of PGE2 in the culture media, Abstract;
Anti-inflammatory effects of 6′-O-acetyl mangiferin from Iris rossii Baker via NF-κb signal blocking in lipopolysaccharide-stimulated RAW 264.7 cells: J.H. Jang, et al.; Chem. Biol. Interact. 2797, 30303 (2016), Application(s): Determination of PGE2 production, Abstract;
Dimethyl fumarate activates the prostaglandin EP2 receptor and stimulates cAMP signaling in human peripheral blood mononuclear cells: S.E. Fiedler, et al.; Biochem. Biophys. Res. Commun. 475, 19 (2016), Application(s): Supernatant PGE2 was measured, Abstract;
Directly interact with Keap1 and LPS is involved in the anti-inflammatory mechanisms of (-)-epicatechin-3-gallate in LPS-induced macrophages and endotoxemia: Y.S. Chiou, et al.; Free. Radic. Biol. Med. 94, 1 (2016), Application(s): Measurement of prostaglandin E2 (PGE2) level in culture medium, Abstract;
Effects of Almond- and Olive Oil-Based Docosahexaenoic- and Vitamin E-Enriched Beverage Dietary Supplementation on Inflammation Associated to Exercise and Age: X. Capo, et al.; Nutrients 8, 619 (2016), Application(s): Cytokine, Eicosanoids and Adhesion Molecules Determination, plasma, Abstract; Full Text
Enhanced anti-inflammatory activity of brown seaweed Laminaria japonica by fermentation using Bacillus subtilis: H.T.V. Lin, et al.; Process Biochem. 51, 1945 (2016), Application(s): Measurement of prostaglandin E2 (PAGE2), cell supernatant,
Enhanced biocompatible and hemocompatible nano/micro porous surface as a biological scaffold for functionalizational and biointegrated implants: S.F. Chu, et al.; J. Alloys Compd. 684, 726 (2016),
Evaluation of anti-acne properties of phloretin in vitro and in vivo: H. Kum, et al.; Int. J. Cosmet. Sci. 38, 85 (2016), Application(s): HaCaT cell culture supernatant, Abstract;
Grid-like surface structures in thermoplastic polyurethane induce anti-inflammatory and anti-fibrotic processes in bone marrow-derived mesenchymal stem cells: Y. Roger, et al.; Colloids Surf. B Biointerfaces 148, 104 (2016), Application(s): ELISA using human bone marrow-derived mesenchymal stem cell supernatant, Abstract;
Hypoxia-mediated impaired erythrocyte Lands' Cycle is pathogenic for sickle cell disease: H. Wu, et al.; Sci. Rep. 6, 29637 (2016), Application(s): ELISA using mouse plasma, Abstract; Full Text
Implantation and pregnancy outcome of Sprague-Dawley rats fed with low and high salt diet: G.O. Oludare, et al.; Middle East Fertil. Soc. J. 21, 228 (2016), Application(s): Measured rat serum PGE2 levels,
In vitro bioactive properties of phlorotannins recovered from hydrothermal treatment of Sargassum muticum: M.P. Casas, et al.; Sep. Purif. Technol. 167, 117 (2016), Application(s): Determination of the amount of PGE2 in thawed plasma supernatants,
Isolation and Identification of a Flavone Apigenin from Marine Red Alga Acanthophora spicifera with Antinociceptive and Anti-Inflammatory Activities: G.A. El Shoubaky, et al.; J. Exp. Neurosci. 10, 21 (2016), Application(s): TNF-α measured in mouse blood samples, Abstract; Full Text
Local intra-uterine Ang-(1-7) infusion attenuates PGE2 and 6-keto PGF1α in decidualized uterus of pseudopregnant rats: K.B. Brosnihan, et al.; Reprod. Biol. Endocrinol. 14, 68 (2016), Application(s): Assaying tissue extracts, Abstract; Full Text
MRI-Based Assessment of Intralesional Delivery of Bone Marrow-Derived Mesenchymal Stem Cells in a Model of Equine Tendonitis: A. Scharf, et al.; Stem Cells Int. 2016, 1 (2016), Application(s): Assessing the ability of MSCs to modulate the inflammatory response, Abstract; Full Text
Resokaempferol-mediated anti-inflammatory effects on activated macrophages via the inhibition of JAK2/STAT3, NF-κB and JNK/p38 MAPK signaling pathways: Q. Yu, et al.; Int. Immunopharmacol. 38, 104 (2016), Application(s): PGE2 was measured in mouse supernatant, Abstract;
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